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OP38 Anti-Pan-Ras (Ab-1) Mouse mAb (DWP)

This Anti-Pan-Ras (Ab-1) Mouse mAb (DWP) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of Pan-Ras (Ab-1).

Anti-Pan-Ras (Ab-1) Mouse mAb (DWP) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP38
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~21 kDa H-, K-, and N-Ras proteins.
Catalogue NumberOP38
Brand Family Calbiochem®
References
ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
Ng, S., et al. 1991. Hybridoma 10, 467.
Hamer, P.J. et al. 1990. Hybridoma 9, 573.
Pullano, T.G., et al. 1989. Oncogene 4, 1003.
Bos, J.L., 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone
Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
Specificity
Wang, Z., et al. 1997. J. Peptide Res. 50, 483.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
CloneDWP
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP38 0

Documentation

Anti-Pan-Ras (Ab-1) Mouse mAb (DWP) Certificates of Analysis

TitleLot Number
OP38

References

Reference overview
Hamer, P.J., et al. 1991 Oncogene 6, 1609.
Ng, S., et al. 1991. Hybridoma 10, 467.
Hamer, P.J. et al. 1990. Hybridoma 9, 573.
Pullano, T.G., et al. 1989. Oncogene 4, 1003.
Bos, J.L., 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-September-2010 JSW
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c x C57BL/6 mice with the specified immunogen and fusing splenocytes with Sp2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.
BackgroundThe human ras gene family consists of three identified members H, K and N-ras encoding proteins of 188-189 amino acids and 21,000 (p21) daltons weight. Human H-and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes are referred to as proto-oncogenes, and have the potential for activation to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of ras proto-oncogene proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and its relevance to human disease diagnosis and prognosis remains to be determined.
HostMouse
Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
CloneDWP
IsotypeIgG2b
Specieshuman, mouse, rat
Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
Ng, S., et al. 1991. Hybridoma 10, 467.
Hamer, P.J. et al. 1990. Hybridoma 9, 573.
Pullano, T.G., et al. 1989. Oncogene 4, 1003.
Bos, J.L., 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Application referencesOriginal Clone
Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
Specificity
Wang, Z., et al. 1997. J. Peptide Res. 50, 483.