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OP38 Anti-Pan-Ras (Ab-1) Mouse mAb (DWP)

OP38
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~21 kDa H-, K-, and N-Ras proteins.

Catalogue NumberOP38
Brand Family Calbiochem®
References
ReferencesHamer, P.J., et al. 1991 Oncogene 6, 1609.
Ng, S., et al. 1991. Hybridoma 10, 467.
Hamer, P.J. et al. 1990. Hybridoma 9, 573.
Pullano, T.G., et al. 1989. Oncogene 4, 1003.
Bos, J.L., 1988. Mutation Res. 195, 255.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlK-Ras Val¹² Protein (Cat. No. WA04)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone
Carney, W.P. et al. 1986. Proc. Natl. Acad. Sci., USA 83, 7485.
Specificity
Wang, Z., et al. 1997. J. Peptide Res. 50, 483.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application CommentsRecognizes H-, K-, and N-Ras proteins. Per Wang, Z., et al. 1997, this clone may also react with other Ras mutants with a hydrophobic amino acid (V, A, C, H, I, L, M, Y, T, N, Q) at position 12. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 5-16 of Ras (H, K, or N) with Val at position 12
CloneDWP
HostMouse
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
OP38 0