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NE1017 Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311)

This Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311) is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Pan-Neuronal Neurofilament Marker.

Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NE1017
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MaMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes ~180-200 kDa pan-neuronal neurofilament marker in rat central nervous system cytoskeletal preparations.
Catalogue NumberNE1017
Brand Family Calbiochem®
Application Data
Detection of rat pan-neuronal neurofilament marker by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311) (Cat. No. NE1017) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
References
ReferencesAgostino, A., et al. 2003. Hum. Mol. Genet. 12, 399.
Tsunoda, I., et al. 2003. Am. J. Pathol. 162, 1259.
Ulfig, N., et al. 1998. Cell Tissue Res. 291, 433.
Product Information
FormLiquid
FormulationUndiluted ascites.
Positive controlRat brain or rat CNS cytoskeletal preparations
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Application CommentsThis antibody cocktail was selected to provide a specific marker for neurons in tissue sections and cultured cells. In contrast to individual anti-nonphosphoneurofilament antibodies that identify different subsets of neurons, this cocktail is a convenient marker for neurons in general and their differentiation from non-neuronal cells. Also useful as an early marker of neuronal migration and differentiation in human fetal development, yielding Golgi-like images without the disadvantages of the lack of selectivity and poor specificity of the Golgi technique. Can be used to trace the "inside-out gradient" of neuron production and differentiation in specifically delineating cell bodies and dendrites. Certain pathologic conditions, such as malnutrition, affect the SMI-311-visualized soma size and dendritic arborization. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin's fixative. This antibody does not react well with tissues or cells fixed in glutaraldehyde/paraformaldehyde. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min to expose the epitope. Trypsin pre-treatment will abolish reactivity. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections and thick tissues sections that have been fixed in 4% paraformaldehyde or cultured cells. For tissues that have not been paraffin-embedded and have been stored for long periods of time in formaldehyde fixatives, it is recommended that the tissues (up to 0.5 cm thick) be boiled in Tris-buffered saline, pH 9.0 for 15 min prior to sectioning. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhomogenized hypothalami extracted from Fischer 344 rat brain
ImmunogenRat
CloneSMI-311
HostMouse
IsotypeIgG₁, IgM cocktail
Species Reactivity
  • Mammals
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NE1017 0

Documentation

Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311) Certificates of Analysis

TitleLot Number
NE1017

References

Reference overview
Agostino, A., et al. 2003. Hum. Mol. Genet. 12, 399.
Tsunoda, I., et al. 2003. Am. J. Pathol. 162, 1259.
Ulfig, N., et al. 1998. Cell Tissue Res. 291, 433.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-October-2007 RFH
ApplicationELISA (1:1000)
Frozen Sections (1:1000, see comments)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000, see comments)
Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Application Data
Detection of rat pan-neuronal neurofilament marker by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Pan-Neuronal Neurofilament Marker Mouse mAb (SMI-311) (Cat. No. NE1017) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~180-200 kDa pan-neuronal neurofilament marker protein.
BackgroundPan-neuronal neurofilament marker is a specific marker for neurons.
HostMouse
Immunogen speciesRat
Immunogenhomogenized hypothalami extracted from Fischer 344 rat brain
CloneSMI-311
IsotypeIgG₁, IgM cocktail
Speciesmammalian
Positive controlRat brain or rat CNS cytoskeletal preparations
FormLiquid
FormulationUndiluted ascites.
Preservative≤0.1% sodium azide
CommentsThis antibody cocktail was selected to provide a specific marker for neurons in tissue sections and cultured cells. In contrast to individual anti-nonphosphoneurofilament antibodies that identify different subsets of neurons, this cocktail is a convenient marker for neurons in general and their differentiation from non-neuronal cells. Also useful as an early marker of neuronal migration and differentiation in human fetal development, yielding Golgi-like images without the disadvantages of the lack of selectivity and poor specificity of the Golgi technique. Can be used to trace the "inside-out gradient" of neuron production and differentiation in specifically delineating cell bodies and dendrites. Certain pathologic conditions, such as malnutrition, affect the SMI-311-visualized soma size and dendritic arborization. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin's fixative. This antibody does not react well with tissues or cells fixed in glutaraldehyde/paraformaldehyde. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH2O for 10 min to expose the epitope. Trypsin pre-treatment will abolish reactivity. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections and thick tissues sections that have been fixed in 4% paraformaldehyde or cultured cells. For tissues that have not been paraffin-embedded and have been stored for long periods of time in formaldehyde fixatives, it is recommended that the tissues (up to 0.5 cm thick) be boiled in Tris-buffered saline, pH 9.0 for 15 min prior to sectioning. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesAgostino, A., et al. 2003. Hum. Mol. Genet. 12, 399.
Tsunoda, I., et al. 2003. Am. J. Pathol. 162, 1259.
Ulfig, N., et al. 1998. Cell Tissue Res. 291, 433.