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454578 Anti-Pan-NMDA NR1 Mouse mAb (R1JHL)

This Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) is validated for use in Immunoblotting for the detection of Pan-NMDA NR1.

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
454578
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
454578-15UG Plastová ampulka 15 μg
Description
OverviewRecognizes the ~115 kDa NMDA receptor NR1 subunit in rat cortex tissue extracts.
Catalogue Number454578
Brand Family Calbiochem®
References
ReferencesLaurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
Monyer, H., et al. 1992. Science 256, 1217.
Product Information
FormLyophilized
FormulationLyophilized from cell culture supernatant.
Positive controlRat cortex tissue extracts
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:300, colorimetric)
Immunoprecipitation (3 µg/200 µg lysate)
Application CommentsDoes not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
ImmunogenRat
CloneR1JHL
HostMouse
IsotypeIgG
Species Reactivity
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
454578-15UG 04055977204155

Documentation

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Pan-NMDA NR1 Mouse mAb (R1JHL) Certificates of Analysis

TitleLot Number
454578

References

Reference overview
Laurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
Monyer, H., et al. 1992. Science 256, 1217.

Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-August-2008 JSW
ApplicationImmunoblotting (1:300, colorimetric)
Immunoprecipitation (3 µg/200 µg lysate)
DescriptionMouse monoclonal antibody lyophilized from cell culture supernatant. Recognizes the ~115 kDa subunit of the NMDAR (N-methyl-D-aspartate receptor) protein.
BackgroundThe rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. NR1 can form NMDA-activated channels when expressed in oocytes, but the currents are larger when combined with one or more of the NMDAR2 (NR2 A-D) subunits.
HostMouse
Immunogen speciesRat
Immunogena recombinant fusion protein consisting of amino acids 1-564 of rat NMDA NR1
CloneR1JHL
IsotypeIgG
Speciesrat
Positive controlRat cortex tissue extracts
FormLyophilized
FormulationLyophilized from cell culture supernatant.
PreservativeNone
CommentsDoes not cross-react with other NMDAR subunits. For immunoblotting, dilute in TBST containing 5% nonfat milk and 0.05% sodium azide. Antibody may be used up to 5 times in immunoblotting experiments without significant loss in signal strength. Store diluted antibody at 4°C between uses. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute in 50 µl PBS (137 mM NaCl, 7.5 mM Na₂HPO₄, 2.7 mM KCl, 1.5 mM KH₂P0₄, pH 7.4)Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesLaurie, D.J., and Seeburg, P.H. 1994. J. Neurosci. 14, 3180
Ishii, T., et al. 1993. J. Biol. Chem. 268, 2836.
Monyer, H., et al. 1992. Science 256, 1217.