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ABN2246-25UL Anti-PTP zeta/Phosphacan

Anti-phosphacan, Cat. No. ABN2246 is a rabbit polyclonal antibody that has been tested for use in Immunohistochemistry (Paraffin) for the detection of Receptor-type tyrosine-protein phosphatase zeta.

Anti-PTP zeta/Phosphacan MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Ptprz1, Ptprz, Ptpz UniProt Number: Q62656
ABN2246-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RIH(P)RbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABN2246-25UL
DescriptionAnti-PTP zeta/Phosphacan
Alternate Names
  • Receptor-type tyrosine-protein phosphatase zeta
  • EC:3.1.3.48
  • R-PTP-zeta
  • 3F8 chondroitin sulfate proteoglycan
  • 3H1 keratan sulfate proteoglycan
  • Phosphacan
Background InformationReceptor-type tyrosine-protein phosphatase zeta (UniProt: Q62656; also known as EC:3.1.3.48, R-PTP-zeta, 3F8 chondroitin sulfate proteoglycan, 3H1 keratan sulfate proteoglycan, Phosphacan) is encoded by the Ptprz1 (also known as Ptprz, Ptpz) gene (GeneID: 25613) in rat. It is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth and negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. It is required for normal differentiation of the precursor cells into mature, fully myelinating oligodendrocytes. RPTPzeta has an extracellular domain (aa 25-1637), a transmembrane domain (aa 1638-1663) and a cytoplasmic domain (aa 1664-2316). It is produced with a signal peptide (aa 1-24) that is cleaved to generate the mature form.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.02% sodium azide.
Applications
ApplicationAnti-phosphacan, Cat. No. ABN2246 is a rabbit polyclonal antibody that has been tested for use in Immunohistochemistry (Paraffin) for the detection of Receptor-type tyrosine-protein phosphatase zeta.
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A representative lot detected PTP zeta/Phosphacan in E16 rat spinal cord (Milev, P., et. al. (1994). J Cell Biol. 127(6 Pt 1):1703-15), rat brain (Haunso, A., et. al. (1999). Brain Res. 834(1-2):219-22), and 21-day postnatal rat retina, 2-week postnatal rat cerebellum, E13 rat spinal cord (Milev, P., et. al. (1998). J Biol Chem. 273(12):6998-7005).
Biological Information
ImmunogenFull length (aa 1-2316) purified Receptor-type tyrosine-protein phosphatase zeta (Phosphacan) isolated from 7-day fetal rat brain.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects PTP zeta/Phosphacan in rat brain tissue.
IsotypeIgG
Species Reactivity
  • Rat
Species Reactivity NoteRat.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Ptprz1
  • Ptprz
  • Ptpz
Purification MethodUnpurified
UniProt Number
Molecular Weight255.34 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in rat cerebral cortex and rat hippocampus tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected PTP zeta/Phosphacan in rat cerebral cortex and rat hippocampus tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN2246-25UL 04054839499210