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MABS1680 Anti-PTEN-alpha Antibody, clone 3A4.1

Detect PTEN-alpha using this mouse monoclonal Anti-PTEN-alpha, clone 3A4.1 Antibody, Cat. No. MABS1680, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PTEN-alpha Antibody, clone 3A4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: PTEN, 10q23del, BRRS, BZS, CWS1, GLM2, MMAC1, MHAM, TEP1 UniProt Number: P60484-2
MABS1680
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1680
DescriptionAnti-PTEN-alpha Antibody, clone 3A4.1
Alternate Names
  • Phosphatidylinositol 3
  • 4
  • 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN-alpha
  • Mitochondrial PTENalpha
  • Phosphatase and tensin-like protein-alpha
  • Mutated in multiple advanced cancers 1-alpha
  • Mitochondrial phosphatase and tensin protein alpha
  • MMAC1 phosphatase and tensin homolog deleted on chromosome 10-alpha
Background InformationPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC 3.1.3.16, EC 3.1.3.48, EC 3.1.3.67; UniProt P60484; also known as Mitochondrial PTENalpha, Phosphatase and tensin-like protein, Mutated in multiple advanced cancers 1, Mitochondrial phosphatase and tensin protein alpha, phosphatase and tensin homolog deleted on chromosome 10) is encoded by the PTEN (also known as 10q23del, BRRS, BZS, CWS1, GLM2, MMAC1, MHAM, TEP1) gene (Gene ID 5728) in human. PTEN is a tumor suppressor that catalyzes the removal of phosphate from phosphoserine, phosphothreonine, and phosphotyrosine residues on substrate proteins, as well as the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. PTEN plays a major role in controlling cell growth and proliferation, and PTEN gene mutains are found in a large number of cancers at high frequency. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylations of several Ser/Thr (S/T) residues in its unfolded C-terminal tail region (PTEN-Ctail, residues 351 403). Alternative translation start site 519 base pairs upstream of the ATG initiation sequence results in the translational variant of PTEN-alpha (PTEN-Long), including additional 173 N-terminal amino acids to the canonical 403-a.a. PTEN sequence. PTEN-Long is a membrane-permeable phosphatase secreted by PTEN-Long producing cells and can enter other cells as an exogenous PI3K signaling-inhibitory agent. PTEN-Long is reported to induce tumor cell death in vitro and in vivo.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationDetect PTEN-alpha using this mouse monoclonal Anti-PTEN-alpha, clone 3A4.1 Antibody, Cat. No. MABS1680, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PTEN-alpha in human cerebellum, pancreas, and small intestine tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human PTEN-alpha N-terminal fragment.
Clone3A4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3A4.1 targets an N-terminal region specific to the spliced isoform alpha (PTEN-Long; P60484-2) and not present in spliced isoform 1 (a.k.a. 55 kDa isoform) or isoform 3.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PTEN
  • 10q23del
  • BRRS
  • BZS
  • CWS1
  • GLM2
  • MMAC1
  • MHAM
  • TEP1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~78 kDa observed. 64.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PTEN-alpha in 10 µg of MCF-7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1680 04054839049644

Documentation

Anti-PTEN-alpha Antibody, clone 3A4.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-PTEN-alpha Antibody, clone 3A4.1 Certificates of Analysis

TitleLot Number
Anti-PTEN-alpha, clone 3A4.1 - 3172414 3172414
Anti-PTEN-alpha, clone 3A4.1 - 3291379 3291379
Anti-PTEN-alpha, clone 3A4.1 - 3321799 3321799
Anti-PTEN-alpha, clone 3A4.1 - 3469434 3469434
Anti-PTEN-alpha, clone 3A4.1 - 3596227 3596227
Anti-PTEN-alpha, clone 3A4.1 - 4025989 4025989
Anti-PTEN-alpha, clone 3A4.1 -Q2735573 Q2735573
Anti-PTEN-alpha, clone 3A4.1 Monoclonal Antibody 3083566