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PC367 Anti-PL-Scramblase (309-317) Rabbit pAb

Anti-PL-Scramblase (309-317) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phospholipid Scramblase

PC367
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





This product has been discontinued.





Recognizes the ~50 kDa (apparent MW) PL-Scramblase protein in colon carcinoma and normal skin tissue.
Catalogue NumberPC367
Brand Family Calbiochem®
SynonymsAnti-Phospholipid Scramblase
References
ReferencesBevers, E.M., et al. 1998. Lupus 7 Suppl 2, S126.
Dekkers, D.W., et al. 1998. Blood 91, 2131.
Stout, J.G., et al. 1998. Biochemistry 37, 14860.
Zhao, J, et al. 1998. Biochemistry 37, 6361.
Zhou, Q., et al. 1998. Blood 92, 1707.
Zhou, Q., et al. 1998. Biochemistry 37, 2356.
Zhou, Z., et al. 1997. J. Biol. Chem. 272, 18240.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlNormal rabbit IgG
Positive controlColon carcinoma or normal skin tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunoblotting Yu, a., et al. 2004. Biochem. J. 381, 609.
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, heat or pressure cooker pre-treatment required)
Application CommentsThe calculated molecular weight is ~35, but migrates at ~50 kDa on SDS-PAGE. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 309-317 of human PL-scramblase
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC367 0

Documentation

Anti-PL-Scramblase (309-317) Rabbit pAb Certificates of Analysis

TitleLot Number
PC367

References

Reference overview
Bevers, E.M., et al. 1998. Lupus 7 Suppl 2, S126.
Dekkers, D.W., et al. 1998. Blood 91, 2131.
Stout, J.G., et al. 1998. Biochemistry 37, 14860.
Zhao, J, et al. 1998. Biochemistry 37, 6361.
Zhou, Q., et al. 1998. Blood 92, 1707.
Zhou, Q., et al. 1998. Biochemistry 37, 2356.
Zhou, Z., et al. 1997. J. Biol. Chem. 272, 18240.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
SynonymsAnti-Phospholipid Scramblase
ApplicationImmunoblotting (1 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, heat or pressure cooker pre-treatment required)
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~50 kDa (apparent MW) PL-Scramblase protein.
BackgroundPhospholipid scramblase (PL-scramblase) is a ~35 kDa protein that mediates the accelerated transbilayer migration of plasma membrane phospholipid in both injured and apoptotic cells. The activity of PL-scramblase and the transmembrane movement is Ca2+ dependent/ATP independent and has a marked preference for phosphatidyl serine. Other cations have been shown to substitute for Ca2+ notably Tb3+ and La3+. Both the murine and the human genes have been cloned. One key difference between the two is the short exocytoplasmic tail in the human protein compared to a membrane inserted segment in the mouse protein. Palmitoylation of PL-scramblase is required for anchoring in the cell membrane where it is localized. Notable is the activation and upregulation of PL-scramblase in response to apoptotic signals early in the apoptotic response. This activation and upregulation is required for the subsequent appearance of phosphatidyl serine on the external membrane surface of apoptotic cells. PL-scramblase also has a role in disease; a defect in the ability to activate the PL-scramblase pathway is the basis for Scott syndrome, an inherited bleeding disorder.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 309-317 of human PL-scramblase
IsotypeIgG
Specieshuman, mouse
Positive controlColon carcinoma or normal skin tissue
Negative controlNormal rabbit IgG
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe calculated molecular weight is ~35, but migrates at ~50 kDa on SDS-PAGE. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesBevers, E.M., et al. 1998. Lupus 7 Suppl 2, S126.
Dekkers, D.W., et al. 1998. Blood 91, 2131.
Stout, J.G., et al. 1998. Biochemistry 37, 14860.
Zhao, J, et al. 1998. Biochemistry 37, 6361.
Zhou, Q., et al. 1998. Blood 92, 1707.
Zhou, Q., et al. 1998. Biochemistry 37, 2356.
Zhou, Z., et al. 1997. J. Biol. Chem. 272, 18240.
Application referencesImmunoblotting Yu, a., et al. 2004. Biochem. J. 381, 609.