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06-578-I-100UL Anti-PI4-Kinase β

Anti-PI4-Kinase β , Cat. No. 06-578-I, is a rabbit polyclonal antibody that detects Phosphatidylinositol 4-kinase b and is tested for use in Immunocytochemistry and Western Blotting.

06-578-I-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue Number06-578-I-100UL
DescriptionAnti-PI4-Kinase β
Alternate Names
  • Phosphatidylinositol 4-kinase beta
  • EC:2.7.1.67
  • PI4K-beta
  • PI4Kbeta
  • PtdIns 4-kinase beta
  • NPIK
  • PI4K92
  • PI4KIII
Background InformationPhosphatidylinositol 4-kinase beta (UniProt: Q9UBF8; also known as EC:2.7.1.67, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KIII) is encoded by the PI4KB (also known as PIK4CB) gene (Gene ID: 5298) in human. A group of four enzymes make up the mammalian phosphatidylinositol (PI) 4-kinase family are divided into two groups based on primary sequence and biochemical properties: consisting of type II (PI4KII and PI4KIIβ) and the type III (PI4KIII and PI4KIIIβ) isozymes. They all catalyze the phosphorylation of phosphatidylinositol (PI) at the D4 position of the inositol head-group to synthesize phosphatidylinositol 4-phosphate (PI4P) that serves as an essential precursor to produce PIP2 and PIP3. PI4-kinase b is a type III peripheral membrane protein that is present in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. It phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It also regulates Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and is involved in Golgi-to-plasma membrane trafficking. PI4-kinase b is shown to be critical for glucose-induced insulin secretion due to its capacity to regulate the release of secretory granules. In addition, it inhibits the insulin-stimulated translocation of glucose transporter-4 in adipocytes through its interaction with neuronal calcium sensor. (Ref.: Chalupska, D., et al. (2019). Sci. Rep. 9; article 567; Clyton, EL., et al. (2013). Prog. Lipid Res. 52(3); 294-304).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-PI4-Kinase β , Cat. No. 06-578-I, is a rabbit polyclonal antibody that detects Phosphatidylinositol 4-kinase b and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in HeLa and SH-SY5Y cell lysates.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Phosphatidylinositol 4-kinase b (PI4-Kinase b).
EpitopeN-terminal
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Phosphatidylinositol 4-kinase b (PI4-kinase b). It targets an epitope within 18 amino acids from the N-terminal region.
IsotypeIgG
Species Reactivity
  • Human
  • Monkey
Species Reactivity NoteHuman, Monkey. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • PI4KB
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~ 92 kDa observed; 91.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PI4-Kinase β in Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-578-I-100UL 04065267463674