Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

04-825 Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal

This Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ.

Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: PDGF-R-beta, CD140B, JTK12, PDGFR, CD140b, PDGFR1, PDGFRB UniProt Number: P09619
04-825
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HIP, WBRbCulture SupernatantMonoclonal Antibody
Description
Catalogue Number04-825
Replaces05-825
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal
References
Product Information
FormatCulture Supernatant
Control
  • RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
PresentationCultured supernantant in 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationThis Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesWesterm Blot Analysis:
A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.

Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate.
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β.
Clone4A56
HostRabbit
SpecificityPDGFRβ, does not cross-react with EGF receptor
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
Gene Symbol
  • PDGF-R-beta
  • CD140B
  • JTK12
  • PDGFR
  • CD140b
  • PDGFR1
  • PDGFRB
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P09619 # Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
SIZE: 1106 amino acids; 123968 Da
SUBUNIT: Homodimer, and heterodimer with PDGFRA. Interacts with APS. The autophosphorylated form interacts directly with SHB and with PIK3C2B, maybe indirectly.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P09619 # A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML). & A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia. & A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1. & A chromosomal aberration involving PDGFRB is a cause in many instances of chronic myeloproliferative disorder with eosinophilia (MPE) [MIM:131440]. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein. & A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP- PDGFRB fusion protein.
SIMILARITY: SwissProt: P09619 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight190kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-825 04053252469763

Documentation

Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal MSDS

Title

Safety Data Sheet (SDS) 

Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal Certificates of Analysis

TitleLot Number
Anti-PDGFR, clone 4A56 - 2341100 2341100
Anti-PDGFR, clone 4A56 - DAM1561706 DAM1561706
Anti-PDGFRβ, clone 4A56 - 2392255 2392255
Anti-PDGFRβ, clone 4A56 - 3521055 3521055
Anti-PDGFRβ, clone 4A56 Monoclonal Antibody 3140996
Anti-PDGFRβ, clone 4A56 Monoclonal Antibody 3086142
Anti-PDGFRβ, clone 4A56 Monoclonal Antibody 2956754
Anti-PDGFRβ, clone 4A56 Monoclonal Antibody 2867094