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MABN2250-25UG Anti-PDE6a Antibody, clone 7A11.1

Anti-PDE6a, clone 7A11.1, Cat. No. MABN2250, is a highly specific mouse monoclonal antibody that targets cGMP-PDE alpha and has been tested for use in Western Blotting.

Anti-PDE6a Antibody, clone 7A11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: PDE6A, PDEA UniProt Number: P16499
MABN2250-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2250-25UG
DescriptionAnti-PDE6a Antibody, clone 7A11.1
Alternate Names
  • Rod cGMP-specific 3
  • 5'-cyclic phosphodiesterase subunit alpha
  • EC: 3.1.4.35
  • cGMP-PDE alpha
  • PDE V-B1
Background InformationRod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (UniProt: P16499; also known as EC: 3.1.4.35, cGMP-PDE alpha, PDE V-B1) is encoded by the PDE6A (also known as PDEA) gene (Gene ID: 5145) in human. cGMP-PDE alpha is expressed in cells of the retinal rod outer segment. cGMP-PDE participates in processes of transmission and amplification of the visual signal. This holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by cGMP-PDE alpha, cGMP hydrolysis, and guanylate cyclase cGMP synthesis. cGMP-PDE alpha has five metal ion binding sites that bind two divalent metal cations per subunit. Site 1 preferentially binds zinc ions, while site 2 has a preference for magnesium and/or manganese ions. cGMP-PDE alpha has two GAF domains (aa 73-222 GAF1 and aa 254-431 GAF2). Mutations in PDE6A gene have been linked to retinitis pigmentosa that is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PDE6a, clone 7A11.1, Cat. No. MABN2250, is a highly specific mouse monoclonal antibody that targets cGMP-PDE alpha and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 77 amino acids from the N-terminal region of human Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (cGMP-PDE alpha).
Clone7A11.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7A11.1 is a mouse monoclonal antibody that specifically detects Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (cGMP-PDE alpha) in human retinal tissue. It targets an epitope within 77 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDE6A
  • PDEA
Purification MethodProtein G purified
UniProt Number
Molecular Weight~100 kDa observed; 99.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retinal tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PDE6a in Human retinal tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2250-25UG 04054839469046