Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

512739 Anti-PARP (215-228) Rabbit pAb

512739
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~110-116 kDa full length PARP protein and the ~85 kDa cleaved fragment of PARP.
  • Antibody Target Gene Symbol: PARP1
  • Target Synonym: 5830444G22Rik, Adprp, Adprp1, ADPRT, ADPRT1, AI893648, C80510, MGC93658, PADPRT, pADPRT-1, PARP, PARS, POLY(ADP-RIBOSE) POLYMERASE 1, PPOL, sPARP-1
  • Entrez Gene Name: poly (ADP-ribose) polymerase 1
  • Hu Entrez ID: 142 142 (Related Antibodies: AM30, PC100, AM68, 512738, 512737, and 512734)
  • Mu Entrez ID: 11545
  • Rat Entrez ID: 25591
  • Catalogue Number512739
    Brand Family Calbiochem®
    References
    Product Information
    FormLiquid
    FormulationUndiluted serum.
    Preservative0.2% sodium azide
    Applications
    Key Applications Enzyme-Linked Immunosorbent Assay
    Immunoblotting (Western Blotting)
    Immunofluorescence
    Application NotesELISA (1:210,000)
    Immunofluorescence (1:50)
    Immunoblotting (1:2000-1:5000, chemiluminescence)
    Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
    Biological Information
    Immunogena synthetic peptide (GVDEVAKKKSKKEK) corresponding to amino acids 215-228 of human poly(ADP-ribose) polymerase
    ImmunogenHuman
    HostRabbit
    IsotypeIgG
    Physicochemical Information
    Dimensions
    Materials Information
    Toxicological Information
    Safety Information according to GHS
    Safety Information
    Product Usage Statements
    Storage and Shipping Information
    Ship Code Dry Ice Only
    Toxicity Standard Handling
    Storage ≤ -70°C
    Avoid freeze/thaw Avoid freeze/thaw
    Do not freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Packaging Information
    Transport Information
    Supplemental Information
    Specifications
    Global Trade Item Number
    Catalogue Number GTIN
    512739 0

    Documentation

    Anti-PARP (215-228) Rabbit pAb MSDS

    Title

    Safety Data Sheet (SDS) 

    Anti-PARP (215-228) Rabbit pAb Certificates of Analysis

    TitleLot Number
    512739

    Brochure

    Title
    Caspases and other Apoptosis Related Tools Brochure
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision08-August-2007 RFH
    ApplicationELISA (1:210,000)
    Immunofluorescence (1:50)
    Immunoblotting (1:2000-1:5000, chemiluminescence)
    DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~110-116 kDa full length PARP protein and the ~85 kDa cleaved fragment of PARP.
    BackgroundDuring apoptosis, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of ~29 kDa and ~85 kDa. This cleavage site is conserved in human, bovine, and chicken PARP. This antibody, and Cat. No. 512738, can be used to discriminate between the necrotic versus apoptotic cleavage of PARP.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (GVDEVAKKKSKKEK) corresponding to amino acids 215-228 of human poly(ADP-ribose) polymerase
    IsotypeIgG
    Specieshuman
    FormLiquid
    FormulationUndiluted serum.
    Preservative0.2% sodium azide
    CommentsVariables associated with assay conditions will dictate the proper working dilution.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling