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517310 Anti-P-Glycoprotein Mouse mAb (C219)

Anti-P-Glycoprotein Mouse mAb (C219) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
517310
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ht, H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
Catalogue Number517310
Brand Family Calbiochem®
References
ReferencesBeck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
Product Information
DeclarationImmunoblotting Lee, G., et al. 2001. J. Pharmacol. Exp. Ther. 299, 204.
FormLiquid
FormulationIn PBS, 1% BSA.
Positive controlCell lines or frozen tissue, such as liver or colon
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Ledoux, S., et al. 2003. Cancer Res. 63, 7284. Veau, C., et al. 2002. J. Pharmacol. Exp. Ther. 302, 742. Hosoyo, K., et al. 2000. AAPS PharmSci. 2, E27.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections
Application NotesFlow Cytometry (5-10 µg/1 x 10⁶ cells)
Frozen Sections (5-10 µg/ml)
Immunoblotting (1-10 µg/ml, see application references, see comments)
Immunocytochemistry (see comments)
Immunoprecipitation (see comments)
Paraffin Sections (1-10 µg/ml)
Application CommentsAlso recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
Biological Information
ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
ImmunogenHamster and Human
CloneC219
HostMouse
IsotypeIgG₁
Species Reactivity
  • Hamster
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
517310 0

Documentation

Anti-P-Glycoprotein Mouse mAb (C219) MSDS

Title

Safety Data Sheet (SDS) 

Anti-P-Glycoprotein Mouse mAb (C219) Certificates of Analysis

TitleLot Number
517310

References

Reference overview
Beck, W.T., et al. 1996. Cancer Res. 56, 3010.
Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.

Citations

Title
  • Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
  • Severine Ledoux, et al. (2003) Glucose depletion enhances P-glycoprotein expression in hepatoma cells: role of endoplasmic reticulum stress response. Cancer Research 63, 7284-7290.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision21-June-2013 JSW
    ApplicationFlow Cytometry (5-10 µg/1 x 10⁶ cells)
    Frozen Sections (5-10 µg/ml)
    Immunoblotting (1-10 µg/ml, see application references, see comments)
    Immunocytochemistry (see comments)
    Immunoprecipitation (see comments)
    Paraffin Sections (1-10 µg/ml)
    DescriptionPurified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 and MDR3 isoforms of P-glycoprotein.
    BackgroundP-glycoprotein is typically expressed in cells with secretory and excretory functions. Its highest expression is in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P-glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P-glycoprotein have also been reported in multidrug-resistant cell lines and in various tumors, sarcomas and lymphomas.
    HostMouse
    Immunogen speciesHamster and Human
    ImmunogenSDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a multidrug resistant human cell line
    CloneC219
    IsotypeIgG₁
    Specieshamster, human, mouse, rat
    Positive controlCell lines or frozen tissue, such as liver or colon
    FormLiquid
    FormulationIn PBS, 1% BSA.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAlso recognizes ~150 kDa sister P-glycoprotein (SPGP). Frozen sections should be fixed in acetone for 10 min at -20°C. Formalin-fixed, paraffin tissue sections should be deparaffinized and rehydrated by conventional means. Boiling samples may reduce visibility of the protein by immunoblotting (see application references). This antibody is also reported to work for immunocytochemistry and immunoprecipitation. A ~200 kDa protein that migrates in the same position as myosin is also seen by immunoblotting. Variables associated with assay conditions will dictate the proper working dilutions.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesBeck, W.T., et al. 1996. Cancer Res. 56, 3010.
    Toth, K., et al. 1992. Am. J. Pathol. 140, 1009.
    Schinkel, A.H., et al. 1991. Cancer Res. 51, 2628.
    Chan, H.S.L., et al. 1988. Lab Invest. 59, 870.
    Citation
  • Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
  • Severine Ledoux, et al. (2003) Glucose depletion enhances P-glycoprotein expression in hepatoma cells: role of endoplasmic reticulum stress response. Cancer Research 63, 7284-7290.
    		•
    Application referencesImmunoblotting Ledoux, S., et al. 2003. Cancer Res. 63, 7284. Veau, C., et al. 2002. J. Pharmacol. Exp. Ther. 302, 742. Hosoyo, K., et al. 2000. AAPS PharmSci. 2, E27.