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PC345 Anti-Orexin A (14-33) (Ab-1) Rabbit pAb

Anti-Orexin A (14-33) (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC345
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes orexin A in mouse and rat brain. Supplied with a control peptide.
Catalogue NumberPC345
Brand Family Calbiochem®
Application Data
Detection of human orexin A by staining frozen sections. Sample: Mouse brain tissue fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-1) Rabbit pAb (Cat. No. PC345) (2 µg/ml). Detection: DAB.
References
ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.
Product Information
FormLiquid
Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide.
Positive controlMouse or rat brain
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunofluorescence
Application NotesELISA (see comments)
Frozen Sections (1-3 µg/ml)
Immunofluorescence (1-4 µg/ml)
Application CommentsThis amino acid sequence is identical to orexin A from mouse, rat, and bovine. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with peptide at 10-6 M. Unconjugated control peptide has been included for use as an internal control for antibody specificity. This antibody has been reported to work for ELISA. This antibody is also available in serum form, Anti-Orexin A (Ab-2) Rabbit pAb (Cat.No. PC362). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsReconstitute peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC345 0

Documentation

Anti-Orexin A (14-33) (Ab-1) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Orexin A (14-33) (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC345

References

Reference overview
De Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationELISA (see comments)
Frozen Sections (1-3 µg/ml)
Immunofluorescence (1-4 µg/ml)
Application Data
Detection of human orexin A by staining frozen sections. Sample: Mouse brain tissue fixed in 4% paraformaldehyde. Primary antibody: Anti-Orexin A (14-33) (Ab-1) Rabbit pAb (Cat. No. PC345) (2 µg/ml). Detection: DAB.
DescriptionPurified rabbit polyclonal antibody. Recognizes orexin A. Supplied with a control peptide.
BackgroundThe hypothalamus has been shown to play a central role in the regulation of energy homeostasis and in the control of feeding behavior. Two homologous peptides have recently been identified for which the message and protein have been localized to the lateral hypothalamus. Orexin A (hypocretin 1), a 33 amino acid peptide, and orexin B (hypocretin 2), a 28 amino acid peptide, are both encoded by a single mRNA transcript. These peptides stimulate food consumption in a dose-dependent manner when administered intracerebroventricularly, and precursor mRNA levels are upregulated upon fasting. In addition to the orexins (hypocretins), two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides. Competitive radioligand binding assays suggest that the orexin receptor-1 is selective for orexin A, while orexin receptor-2 is nonselective for both peptides.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (CRLYELLHGAGNHAAGILTL) corresponding to amino acids 14-33 of human orexin A
IsotypeIgG
Speciesbovine, human, mouse, rat
Positive controlMouse or rat brain
FormLiquid
Formulation100 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin and 25 µg lyophilized control peptide.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide (antibody only)
CommentsThis amino acid sequence is identical to orexin A from mouse, rat, and bovine. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the purified antibody with peptide at 10-6 M. Unconjugated control peptide has been included for use as an internal control for antibody specificity. This antibody has been reported to work for ELISA. This antibody is also available in serum form, Anti-Orexin A (Ab-2) Rabbit pAb (Cat.No. PC362). Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsReconstitute peptide with 25 µl dH₂O for a working stock of 1 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesDe Lecea, L., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 322.
Flier, J.S. and Maratos-Flier, E. 1998. Cell 92, 437.
Sakurai, T., et al. 1998. Cell 92, 573.
Schwartz, M.W. 1998. Nat. Med. 4, 385.