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CB1045 Anti-OCLN Mouse pAb

This Anti-OCLN Mouse pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of OCLN.

Anti-OCLN Mouse pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Occludin

CB1045
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMPolyclonal Antibody
Description
OverviewRecognizes the ~55-60 kDa OCLN protein in HeLa cells and human and mouse liver tissue.
Catalogue NumberCB1045
Brand Family Calbiochem®
SynonymsAnti-Occludin
Application Data
Detection of human OCLN by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse OCLN by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human OCLN by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1:1200) and an anti-CSNK1E mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHuman liver tissue, Mouse liver tissue, HeLa cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1:50-1:1200)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, human OCLN (aa 1-522)
ImmunogenHuman
HostMouse
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1045 0

Documentation

Anti-OCLN Mouse pAb MSDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-November-2010 RFH
SynonymsAnti-Occludin
ApplicationImmunoblotting (1-5 µg/ml)
Immunocytochemistry (1:50-1:1200)
Application Data
Detection of human OCLN by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse OCLN by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human OCLN by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1:1200) and an anti-CSNK1E mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
DescriptionPurifried rabbit polyclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
BackgroundOCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.
HostMouse
Immunogen speciesHuman
ImmunogenFull-length, human OCLN (aa 1-522)
IsotypeIgG
Specieshuman, mouse
Positive controlHuman liver tissue, Mouse liver tissue, HeLa cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review