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MABE1039 Anti-Nup96 Antibody, clone 4H5

Anti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.

Anti-Nup96 Antibody, clone 4H5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: Nup98 UniProt Number: Q6PFD9
MABE1039
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1039
DescriptionAnti-Nup96 Antibody, clone 4H5
Alternate Names
  • Nuclear pore complex protein Nup98-Nup96
  • Nuclear pore complex protein Nup98
  • 98 kDa nucleoporin
  • Nucleoporin Nup98
  • Nup98
  • Nuclear pore complex protein Nup96
  • 96 kDa nucleoporin
  • Nucleoporin Nup96
  • Nup96
  • Nup96
Background InformationThe murine gene Nup98 (Gene ID 269966; also known as AI849286, 4732457F17) encodes the 1816-amino acid nuclear pore complex protein Nup98-Nup96 (UniProt Q6PFD9), which is posttranslationally autocleaved between aa 800 and 801 to form Nup98 (aa 1-800) and Nup96 (aa 801-1816). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. The core structure of the NPC is maintained by the Nup93-205 complex sandwiched between the supporting structures of the Nup107-160 complex on the nuclear and cytoplasmic faces of the pore. Nup96 is a component of the Nup107-160 complex, the largest subunit of the NPC. Nup96 knockout is reported to be fatal in mice and Nup96(+/-) mice with low levels of Nup96 display impaired immune response and are highly susceptible to viral infection.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nup96 Antibody, clone 4H5 is an antibody against Nup96 for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).
Immunocytochemistry Analysis: A representative lot detected Nup96 in NIH/3T3 cells (Mizuguchi, C., et al. (2010). Hybridoma. 29(6):551-553).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to mouse Nup96 near the N-terminus.
EpitopeNear N-terminus.
Clone4H5
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityReacts with Nup98-Nup96 precursor (isoforms 1, 2, and 6), nucleoporin Nup96 (isoform 5), but not nucleoporin Nup98 (isoform 3).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nup98
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~120 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Nup96 in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1039 04055977170689

Documentation

Anti-Nup96 Antibody, clone 4H5 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Nup96 Antibody, clone 4H5 Certificates of Analysis

TitleLot Number
Anti-Nup96, clone 4H5 - 3327585 3327585
Anti-Nup96, clone 4H5 - 3931393 3931393
Anti-Nup96, clone 4H5 - 4110918 4110918
Anti-Nup96, clone 4H5 -Q2573209 Q2573209

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.