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PC344 Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb

Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC344
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
MRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~70 kDa netrin-1 protein in embryonic mouse brain tissue.
Catalogue NumberPC344
Brand Family Calbiochem®
Application Data
Detection of mouse netrin-1 by immunoblotting. Samples: Whole tissue extract from mouse brain harvested at embryonic days 14 (E14) and 16 (E16), postnatal day 1 (P1), and adult (A). Primary antibody: Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb (Cat. No. PC344) (1 μg/ml). Detection: chemiluminescence.
References
ReferencesVarela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev. 11, 545.
Harris, R., et al. 1996. Neuron 17, 217.
Keino-Masu, K., et al. 1996. Cell 87, 175.
Mitchell, K.J., et al. 1996. Neuron 17, 203.
Serafini, T., et al. 1996. Cell 87, 1001.
Tessier-Lavigne, M. and Goodman, C.S. 1996. Science 274, 1123.
Kennedy, T.E., et al. 1994. Cell 78, 425.
Serafini, T., et al. 1994. Cell 78, 409.
Ishii, N., et al. 1992. Neuron 9, 873.
Hedgecock, E.M., et al. 1990. Neuron 4, 61.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlMouse brain tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml)
Frozen Sections (not recommended)
Application CommentsImmunoblotting was performed using brain tissue from various developmental stages. Netrin-1 protein is enriched in embryonic neural tissue. Antibody should be titrated for optimal results in individual systems. For immunohistochemistry applications, Anti-Netrin-1 (Ab-2) Rabbit pAb is also available (Cat. No. PC364-100 µg).
Biological Information
Immunogena synthetic peptide (LGNAEDSPDQSG) corresponding to amino acids 557-568 of mouse netrin-1, conjugated to KLH
ImmunogenMouse
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC344 0

Documentation

Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb Certificates of Analysis

TitleLot Number
PC344

References

Reference overview
Varela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev. 11, 545.
Harris, R., et al. 1996. Neuron 17, 217.
Keino-Masu, K., et al. 1996. Cell 87, 175.
Mitchell, K.J., et al. 1996. Neuron 17, 203.
Serafini, T., et al. 1996. Cell 87, 1001.
Tessier-Lavigne, M. and Goodman, C.S. 1996. Science 274, 1123.
Kennedy, T.E., et al. 1994. Cell 78, 425.
Serafini, T., et al. 1994. Cell 78, 409.
Ishii, N., et al. 1992. Neuron 9, 873.
Hedgecock, E.M., et al. 1990. Neuron 4, 61.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationImmunoblotting (1 µg/ml)
Frozen Sections (not recommended)
Application Data
Detection of mouse netrin-1 by immunoblotting. Samples: Whole tissue extract from mouse brain harvested at embryonic days 14 (E14) and 16 (E16), postnatal day 1 (P1), and adult (A). Primary antibody: Anti-Netrin-1 (Ab-1) (557-568) Rabbit pAb (Cat. No. PC344) (1 μg/ml). Detection: chemiluminescence.
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~60 kDa netrin-1 protein.
BackgroundIn the developing nervous system, axon guidance and the establishment of appropriate neuronal connections involves a number of chemoattractant and chemorepellent molecules. Netrins are related to the extracellular matrix protein laminin and comprise a family of secreted molecules capable of guiding the growth cone. The first netrin family member to be characterized, UNC-6 from C. elegans, was initially identified from the gene mutation which lead to defects in circumferential axon migration in the nematode. In contrast, the identification of the first vertebrate netrins, designated netrin-1 and netrin-2, resulted from a biochemical rather than genetic approach. Netrin homologs have subsequently been identified from a number of species. Results from several studies suggest a role for netrin-1 in mediating chemotropic and outgrowth-promoting activities of floor plate cells to guide commissural axons towards the ventral midline. DCC (Deleted in Colorectal Cancer) is believed to function as a component of a receptor complex which mediates the effects of netrin-1 on commissural axons and helps coordinate the navigation of axons to establish precise patterns of neural connectivity. In addition to the netrins and their receptors, a number of other diffusable or membrane-associated ligand families and their receptors have been implicated in axon guidance, including the semaphorins, ephrins, and cell adhesion molecules.
HostRabbit
Immunogen speciesMouse
Immunogena synthetic peptide (LGNAEDSPDQSG) corresponding to amino acids 557-568 of mouse netrin-1, conjugated to KLH
IsotypeIgG
Speciesmouse
Positive controlMouse brain tissue
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsImmunoblotting was performed using brain tissue from various developmental stages. Netrin-1 protein is enriched in embryonic neural tissue. Antibody should be titrated for optimal results in individual systems. For immunohistochemistry applications, Anti-Netrin-1 (Ab-2) Rabbit pAb is also available (Cat. No. PC364-100 µg).
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesVarela-Echavarria, A., and Guthrie, S. 1997. Genes and Dev. 11, 545.
Harris, R., et al. 1996. Neuron 17, 217.
Keino-Masu, K., et al. 1996. Cell 87, 175.
Mitchell, K.J., et al. 1996. Neuron 17, 203.
Serafini, T., et al. 1996. Cell 87, 1001.
Tessier-Lavigne, M. and Goodman, C.S. 1996. Science 274, 1123.
Kennedy, T.E., et al. 1994. Cell 78, 425.
Serafini, T., et al. 1994. Cell 78, 409.
Ishii, N., et al. 1992. Neuron 9, 873.
Hedgecock, E.M., et al. 1990. Neuron 4, 61.