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MAB5264 Anti-Nerve Growth Factor Receptor (p75) Antibody, clone 8211

MAB5264
50 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IHC, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB5264
Replaces04-1111
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nerve Growth Factor Receptor (p75) Antibody, clone 8211
Alternate Names
  • NGF-Receptor p75
  • Low Affinity NGF Receptor
  • NGFR
Background InformationThe low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.
Product Information
FormatPurified
Control
  • Brain tissue
PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Quality LevelMQ100
Applications
ApplicationAnti-Nerve Growth Factor Receptor (p75) Antibody, clone 8211 is an antibody against Nerve Growth Factor Receptor (p75) for use in IC, IH, IH(P) & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry: 5-10 μg/mL

Immunocytochemistry: 5-10 μg/mL

Immunohistochemistry on paraffin sections: Cattoretti, Blood 81:1726-1738, 1993. http://www.bloodjournal.org/cgi/reprint/81/7/1726.pdf

Western Blot: 1ug/mL

Immunoabsorptive purification of NGF-receptors

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenMembrane fragments of the melanoma cell line WM-9 (Herlyn et al., 1983).
Clone8211
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityReacts with NGF-receptors in human system.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
Gene Symbol
  • NTF3
  • NT3
  • NGF2
  • HDNF
  • MGC129711
  • NGF-2
  • NT-3
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
SIZE: 427 amino acids; 45183 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
Molecular Weight75 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
MAB5264 04053252397073