Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2322-100UL Anti-Nerve Growth Factor Antibody, clone α D11

Anti-Nerve Growth Factor, clone D11, Cat. No. MABN2322, is a rat monoclonal antibody that detects Nerve growth factor and is tested for use in ELISA, Inhibition Assay, Radioimmunoassay, and Surface Plasmon Resonance.

MABN2322-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2322-100UL
DescriptionAnti-Nerve Growth Factor Antibody, clone α D11
Alternate Names
  • NGF
  • beta-NGF
Background InformationBeta-nerve growth factor (UniProt: P01139; also known as Beta-NGF) is encoded by the Ngf (also known as Ngfb) gene (Gene ID: 18049) in murine species. β-NGF, an endogenous neurotrophic factor, is a homodimeric glycoprotein that is synthesized with a signal peptide (aa 1-18) and a propeptide (aa 19-121) that are subsequently cleaved off to generate the mature form (aa 122-241). It is highly expressed in male submaxillary gland and its levels are much lower in female submaxillary gland. Its expression is reported to oscillate in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain. NGF is important for the development and maintenance of the sympathetic and sensory nervous systems. It serves as an extracellular ligand for the NTRK1 and NGF receptors to activate cellular signaling cascades to regulate neuronal proliferation, differentiation, and survival. However, it does not permeate the blood-brain barrier. Its precursor form (ProNGF) is reported to function as a ligand for the heterodimeric receptor formed by Sortilin Related VPS10 Domain Containing Receptor 2 (SORCS2) and NGF receptor that activates signaling cascade leading to inactivation of RAC1 and/or RAC2. In contrast to the mature form, ProNGF is known to promote neuronal apoptosis. N-glycosylation of ProNGF is reported to be essential for its processing, secretion, and construction of the tertiary fold of the β-NGF homodimer. Clone D11 is a potent antagonist that can effectively antagonize rodent and human NGF in a variety of in vitro and in vivo systems. (Ref.: Covaceuszach, S., et al. (2012). PLoS ONE 7(3): e32212; Feng, D., et al. (2010). J. Mol. Biol. 396(4); 967-984; Scott, J., et al. (1983). Nature. 302(5908); 538-540).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-Nerve Growth Factor, clone D11, Cat. No. MABN2322, is a rat monoclonal antibody that detects Nerve growth factor and is tested for use in ELISA, Inhibition Assay, Radioimmunoassay, and Surface Plasmon Resonance.
Key Applications
  • ELISA
  • Surface plasmon resonance
  • Radioimmunoassay
  • Inhibition
Application NotesTested applications

Surface plasmon resonance: A representative lot detected Nerve Growth Factor in Surface plasmon resonance applications (Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

ELISA Analysis: A representative lot detected Nerve Growth Factor in ELISA applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10; Covaceuszach, S., et al. (2012). PLoS One. 7(3):e32212).

Inhibition Assay: A representative lot competitively inhibited the biological activity of mNGF and prevented the binding of NGF to PC12 cells. (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Radioimmunoassay: A representative lot detected Nerve growth factor in Radioimmunoassay applications (Cattaneo, A., et al. (1988). J. Neurochem. 50(4):1003-10).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen2.5S NGF purified from the mouse submandibular glands.
EpitopeN-terminal half
Cloneα D11
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone D11 is a rat monoclonal antibody that detects Nerve growth factor (NGF). It targets an epitope within 23 amino acids from the N-terminal half of the mature NGF.
IsotypeIgG2a
Species Reactivity
  • Chicken
  • Human
  • Mouse
Species Reactivity NoteChicken, Human, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Ngf
Purification MethodProtein G purified
UniProt Number
Molecular Weight27.08 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant mouse Nerve growth factor.

ELISA Analysis (ELISA): A representative lot of this antibody detected various dilution of recombinant mouse Nerve growth factor.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2322-100UL 04065268900734