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ST1633 Anti-NOV Mouse mAb (3C2)

ST1633
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the ~37 kDa NOV protein.
Catalogue NumberST1633
Brand Family Calbiochem®
SynonymsAnti-Nephroblastoma Overexpressed
Application Data
Detection of human NOV by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human NOV (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-NOV Mouse mAb (3C2) (Cat. No. ST1633) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human NOV (aa 1-358), expressed as a GST fusion protein
Clone3C2
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1633 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Nephroblastoma Overexpressed
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human NOV by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length, recombinant, human NOV (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-NOV Mouse mAb (3C2) (Cat. No. ST1633) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa NOV protein.
BackgroundThe protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.
HostMouse
ImmunogenFull-length, recombinant, human NOV (aa 1-358), expressed as a GST fusion protein
Clone3C2
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review