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MABE659 Anti-Myb Antibody, clone 8F12.1

Anti-Myb, clone 8F12.1 Antibody, Cat. No. MABE659, is a highly specific mouse monoclonal antibody that targets Myb and has been tested in Western Blotting.

Anti-Myb Antibody, clone 8F12.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: MYB UniProt Number: P10242
MABE659
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE659
DescriptionAnti-Myb Antibody, clone 8F12.1
Alternate Names
  • Transcriptional activator Myb
  • Proto-oncogene c-Myb
Background InformationTranscriptional activator Myb (UniProt: P10242; also known as Myb, Proto-oncogene c-Myb) is encoded by the MYB gene (Gene ID: 4602) in human. Myb is a transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3' and plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. It is negatively regulated by microRNA-155. It consists of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain, and a C-terminal domain involved in transcriptional repression. MYB gene is aberrantly expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Myb protein is also reported to bind to HTLV-I long terminal repeat (LTR) sequences at four distinct regions in a sequence specific manner and activates LTR-mediated transcription. (Ref.: Dasgupta, P., et al. (1992). J. Virol. 66(1): 270-276).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Myb, clone 8F12.1 Antibody, Cat. No. MABE659, is a highly specific mouse monoclonal antibody that targets Myb and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST/His-taged recombinant protein fragment corresponding to 154 amino acids from the C-terminal region of human Transcriptional activator Myb.
Clone8F12.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8F12.1 specifically detects human Myb protein. It targets an epitope within 154 amino acids in the C-terminal region.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYB
Purification MethodProtein G purified
UniProt Number
Molecular Weight~80 kDa observed; 72.34 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Myb in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE659 04054839197727