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AB1553 Anti-Metabotropic Glutamate Receptor 2/3 Antibody

AB1553
50 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Mk, RIH(P), IHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB1553
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Metabotropic Glutamate Receptor 2/3 Antibody
Alternate Names
  • mGluR2/3
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationAffinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.
Quality LevelMQ100
Applications
ApplicationAnti-Metabotropic Glutamate Receptor 2/3 Antibody detects level of Metabotropic Glutamate Receptor 2/3 & has been published & validated for use in IH(P), IH & WB.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot analysis can be done at final concentrations of 0.1-0.5 μg/mL. AB1553 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.1-0.5 μg/mL.

The antibody has not been tested for use in immunoprecipitation. However, antibodies to the carboxy termini of other glutamate receptors are very effective in immunoprecipitation.

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenCarboxy terminus peptide of rat mGluR2 conjugated to BSA with glutaraldehyde (NGREVVDSTTSSL).
HostRabbit
SpecificityMetabotropic glutamate receptors mGluR2 and mGluR3, which have nearly identical C-termini. There is also a very slight recognition of mGluR5. Western blot analysis of rat brain shows bands migrating at Mr = 100,000 (which contains both mGluR2 and mGluR3) and 190,000. The larger band is thought to be a dimer of the smaller band.
Species Reactivity
  • Monkey
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryL-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
Gene Symbol
  • GRM2
  • mGlu2
  • GPRC1B
  • MGLUR2
  • mGluR2
  • GLUR2
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q14416 # Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
SIZE: 872 amino acids; 95568 Da
SUBUNIT: Interacts with GRASP (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in different regions of the adult brain as well as in fetal brain.
SIMILARITY: SwissProt: Q14416 ## Belongs to the G-protein coupled receptor 3 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB1553 04053252325182

Documentation

Anti-Metabotropic Glutamate Receptor 2/3 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Metabotropic Glutamate Receptor 2/3 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) - 3307482 3307482
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) - 3903070 3903070
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) - 4162073 4162073
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2878312
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2279241 2279241
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY -2584415 2584415
RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY -2772660 2772660

References

Reference overviewApplicationSpeciesPub Med ID
CCL2-ethanol interactions and hippocampal synaptic protein expression in a transgenic mouse model.
Gruol, DL; Vo, K; Bray, JG; Roberts, AJ
Frontiers in integrative neuroscience  8  29  2014

Show Abstract
Western Blotting24772072 24772072
Selective silencing of individual dendritic branches by an mGlu2-activated potassium conductance in dentate gyrus granule cells.
Brunner, J; Ster, J; Van-Weert, S; Andrási, T; Neubrandt, M; Corti, C; Corsi, M; Ferraguti, F; Gerber, U; Szabadics, J
The Journal of neuroscience : the official journal of the Society for Neuroscience  33  7285-98  2013

Show Abstract
23616537 23616537
Altered synaptic transmission in the hippocampus of transgenic mice with enhanced central nervous systems expression of interleukin-6.
Nelson, TE; Olde Engberink, A; Hernandez, R; Puro, A; Huitron-Resendiz, S; Hao, C; De Graan, PN; Gruol, DL
Brain, behavior, and immunity  26  959-71  2011

Show Abstract
Western BlottingMouse22609298 22609298
Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6.
Gruol, DL; Puro, A; Hao, C; Blakely, P; Janneke, E; Vo, K
Journal of neuroimmunology  239  28-36  2010

Show Abstract
Western BlottingMouse21890220 21890220
Type 2 metabotropic glutamate receptor (mGluR2) fails to negatively couple to cGMP in stably transfected cells.
Wroblewska, B; Wegorzewska, IN; Bzdega, T; Neale, JH
Neurochemistry international  58  176-9  2010

Show Abstract
Western Blotting21115084 21115084
Metabotropic glutamate receptor mGlu2 is resistant to homologous agonist-induced desensitization but undergoes protein kinase C-mediated heterologous desensitization.
Lennon SM, Rivero G, Matharu A, Howson PA, Jane DE, Roberts PJ, Kelly E
Eur J Pharmacol  649  29-37. Epub 2010 Sep 15.  2009

Show Abstract
20826132 20826132
Elevated level of metabotropic glutamate receptor 2/3 in the prefrontal cortex in major depression.
Feyissa, AM; Woolverton, WL; Miguel-Hidalgo, JJ; Wang, Z; Kyle, PB; Hasler, G; Stockmeier, CA; Iyo, AH; Karolewicz, B
Progress in neuro-psychopharmacology & biological psychiatry  34  279-83  2009

Show Abstract
Western Blotting19945495 19945495
The mGluR2/3 agonist LY379268 blocks the effects of GLT-1 upregulation on prepulse inhibition of the startle reflex in adult rats.
Bellesi, M; Conti, F
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  35  1253-60  2009

Show Abstract
Immunocytochemistry20072121 20072121
Chronic CXCL10 alters neuronal properties in rat hippocampal culture.
Cho, J; Nelson, TE; Bajova, H; Gruol, DL
Journal of neuroimmunology  207  92-100  2009

Show Abstract
19167097 19167097
Group II metabotropic glutamate receptor activation on peripheral nociceptors modulates TRPV1 function.
Carlton, SM; Du, J; Zhou, S
Brain research  1248  86-95  2009

Show Abstract
Immunohistochemistry19026992 19026992