Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN770 Anti-Melanopsin Antibody, clone 1F8.1

This Anti-Melanopsin Antibody, clone 1F8.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Melanopsin .

Anti-Melanopsin Antibody, clone 1F8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: OPN4, MOP UniProt Number: Q9UHM6
MABN770
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN770
DescriptionAnti-Melanopsin Antibody, clone 1F8.1
Alternate Names
  • Melanopsin
  • Opsin-4
Background InformationMelanopsin (UniProt Q9UHM6; also known as Opsin-4) is encoded by the OPN4 (also known as MOP) gene (Gene ID 94233) in human. Opsins belong to the superfamily of G protein-coupled receptors (GPCRs). Melanopsin is a blue light-sensitive opsin protein that is capable of rendering cells intrinsically light responsive. Melanopsin expression defines a population of retinal ganglion cells that are intrinsically photosensitive (ipRGCs). Melanopsin allows mammals to measure irradiance for centrally mediated responses such as circadian entrainment. In addition, the information generated by melanopsin is used by the visual system as a signal for light adaptation. Using melanopsin-dependent phototransduction mechanism coupled with extrinsic signals originating with rods and cones, ipRGCs can encode light levels over many decimal orders, allowing the visual system to adapt to a broad range of light intensity from starlight to broad daylight.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Melanopsin Antibody, clone 1F8.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Melanopsin .
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Melanopsin in human retina tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Melanopsin C-terminal sequence
EpitopeNear C-terminus
Clone1F8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1F8.1 is expected to react with both spliced isoforms of human melanopsin.
IsotypeIgG2aλ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • OPN4
  • MOP
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~52 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retinal pigment epithelia (RPE) ARPE-19 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Melanopsin in 10 µg of human retinal pigment epithelia (RPE) ARPE-19 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN770 04055977175431

Documentation

Anti-Melanopsin Antibody, clone 1F8.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Melanopsin Antibody, clone 1F8.1 Certificates of Analysis

TitleLot Number
Anti-Melanopsin, clone 1F8.1 - 3202189 3202189
Anti-Melanopsin, clone 1F8.1 - 3336230 3336230
Anti-Melanopsin, clone 1F8.1 - 3819910 3819910
Anti-Melanopsin, clone 1F8.1 -Q2578148 Q2578148

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.