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PC709 Anti-MYH (33-51) Rabbit pAb

This Anti-MYH (33-51) Rabbit pAb is validated for use in Immunoblotting, Immunofluorescence, Paraffin Sections for the detection of MYH (33-51).

Anti-MYH (33-51) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MutY Homolog

PC709
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC709-100UL Sklená flaša 100 ul
Description
OverviewRecognizes the MYH protein and several of its approximately 10 isoforms in PC12, Jurkat, and HeLa cells.
Catalogue NumberPC709
Brand Family Calbiochem®
SynonymsAnti-MutY Homolog
References
ReferencesWang, G., et al. 2000. Nucleic Acids Res. 28, 2135.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlPC12, Jurkat, or HeLa cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections Barouch, L.A., et al. 2006. Circ. Res. 98, 119.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Application NotesImmunoblotting (1:1500)
Immunofluorescence (1:500)
Paraffin Sections (see application references)
Application CommentsDetects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC709-100UL 04055977207880

Documentation

Anti-MYH (33-51) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-MYH (33-51) Rabbit pAb Certificates of Analysis

TitleLot Number
PC709

References

Reference overview
Wang, G., et al. 2000. Nucleic Acids Res. 28, 2135.

Citations

Title
  • Barouch, L.A., et al. 2006. Circulation Research 98, 119.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision15-October-2007 RFH
    SynonymsAnti-MutY Homolog
    ApplicationImmunoblotting (1:1500)
    Immunofluorescence (1:500)
    Paraffin Sections (see application references)
    DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~52-57 kDa isoforms of MYH.
    BackgroundOxidative DNA base lesions such as 8-oxoguanine (8-oxoG) are frequently mutagenic and have been implicated in a variety of pathophysiological states including cancer, Alzheimer's disease, arthritis, and cardiovascular disease. These lesions are generated by oxidative stress. MYH is a mammalian homolog of the E. coli Mut Y DNA glycosylase that excises adenine residues misincorporated opposite the oxidized base, 8-oxoG. MYH up-regulation can be observed following damage in mitochondrial DNA. Single mutations in this protein result in reduced translation efficiency of transcripts. Mutations in MYH have been observed in human colorectal tumors and lung cancer cell lines.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (QEGRQKHAKNNSQAKPSAC) corresponding to amino acids 33-51 of human MYH
    IsotypeIgG
    Specieshuman, rat
    Positive controlPC12, Jurkat, or HeLa cells
    FormLiquid
    FormulationUndiluted serum.
    Preservative≤0.1% sodium azide
    CommentsDetects MYH and several of its approximately ten isoforms. In Jurkat cells the ~52 kDa and ~53 kDa variants were found in the nuclear fraction while the ~57 kDa protein is found in the mitochondria. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesWang, G., et al. 2000. Nucleic Acids Res. 28, 2135.
    Citation
  • Barouch, L.A., et al. 2006. Circulation Research 98, 119.
    		•
    Application referencesParaffin Sections Barouch, L.A., et al. 2006. Circ. Res. 98, 119.