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MAB13426 Anti-MMP-13 Antibody, clone LIPCO IID1

MAB13426
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB13426
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-MMP-13 Antibody, clone LIPCO IID1
Alternate Names
  • Collagenase-3
References
Product Information
FormatPurified
Control
  • POSITIVE CONTROL: Conditioned, serum-free medium from (PMA-treated) rat fibrosarcoma cells or rat keratinocyte CCL-4.
Quality LevelMQ100
Applications
ApplicationAnti-MMP-13 Antibody, clone LIPCO IID1 is an antibody against MMP-13 for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blotting (Lyons, 1991; Lyons, 1993): 1:200-1:400 at room temperature for 2 hours.

Immunofluorescence

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenRat MMP-13 from BC-1 cells.
Clone LIPCO IID1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityIt recognizes proteins of ~60 kDa and ~48 kDa which are identified as pro (latent) and activate forms of matrix metalloproteinase-13 (MMP-13; also known as Collagenase-3). Antibody shows no cross-reactivity with the pro and active forms of other MMPs. Human collagenase-3 (MMP13) is a recently identified member of the matrix metalloproteinase (MMP) family that is expressed in breast carcinomas and in articular cartilage from arthritic patients. The MMP-13 gene has been isolated and characterized. This gene is composed of 10 exons and 9 introns and spans over 12.5 kb. The overall organization of the collagenase-3 gene is similar to that of other MMP genes clustered at chromosome 11q22, including fibroblast collagenase (MMP-1), matrilysin (MMP-7), and macrophage metalloelastase (MMP-12), but is more distantly related to genes coding for stromelysin-3 (MMP-11), gelatinase-A (MMP-2), and gelatinase-B (MMP-9), which map outside of this gene cluster.

Cellular Localization:

Cytoplasmic
IsotypeIgG1
Species Reactivity
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryProteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
Gene Symbol
  • MMP13
  • MMP-13
  • CLG3
  • EC 3.4.24.-
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P45452 # Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.
SIZE: 471 amino acids; 53820 Da
TISSUE SPECIFICITY: Seems to be specific to breast carcinomas.
DOMAIN: SwissProt: P45452 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
DISEASE: SwissProt: P45452 # Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia type 2 (SEMD2) [MIM:602111]; also known as spondyloepimetaphyseal dysplasia type Missouri. SEMDs are a heterogeneous group of skeletal disorders characterized by defective growth and modeling of the spine and long bones. The SEMDs are distinguished from the spondylometaphyseal dysplasias and the spondyloepiphyseal dysplasias by the combined involvement of the epiphyses and metaphyses. The 3 disorders have malformations of the vertebrae in common.
SIMILARITY: Belongs to the peptidase M10A family. & Contains 4 hemopexin-like domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB13426 04053252668395

Documentation

Anti-MMP-13 Antibody, clone LIPCO IID1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-MMP-13 Antibody, clone LIPCO IID1 Certificates of Analysis

TitleLot Number
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) 2464497
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) MONOCLONAL ANTIBODY - 2137984 2137984
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) - 3774202 3774202
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) - 3989963 3989963
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) -2602773 2602773
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) -2698441 2698441
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) -2726139 2726139
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) -2749226 2749226
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) -2845134 2845134
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3) -2845560 2845560

References

Reference overviewPub Med ID
SUMO1 regulates endothelial function by modulating the overall signals in favor of angiogenesis and homeostatic responses.
Yang, Ping, et al.
Am J Transl Res, 5: 427-40 (2013)  2013

Show Abstract
23724166 23724166
Protective effects of allopurinol against acute liver damage and cirrhosis induced by carbon tetrachloride: modulation of NF-κB, cytokine production and oxidative stress.
Liseth R Aldaba-Muruato,Mario G Moreno,Mineko Shibayama,Víctor Tsutsumi,Pablo Muriel
Biochimica et biophysica acta  1820  2011

Show Abstract
22056511 22056511
Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures: role of osteoblast matrix metalloproteinase (MMP)-13 in bone metastasis.
Morrison, C; Mancini, S; Cipollone, J; Kappelhoff, R; Roskelley, C; Overall, C
The Journal of biological chemistry  286  34271-85  2010

Show Abstract
21784845 21784845
Noninvasive assessment of UV-induced skin damage: comparison of optical measurements to histology and MMP expression.
Papazoglou E, Sunkari C, Neidrauer M, Klement JF, Uitto J
Photochem Photobiol  86  138-45. Epub 2009 Nov 10.  2009

Show Abstract
19906094 19906094
Discoidin domain receptor 2 is associated with the increased expression of matrix metalloproteinase-13 in synovial fibroblasts of rheumatoid arthritis.
Jin Su,Jiangtian Yu,Tingting Ren,Wei Zhang,Yuanqiang Zhang,Xinping Liu,Tiezheng Sun,Houshan Lu,Keiji Miyazawa,Libo Yao
Molecular and cellular biochemistry  330  2009

Show Abstract
19415460 19415460
A matrix metalloproteinase-9 activation cascade by hepatic stellate cells in trans-differentiation in the three-dimensional extracellular matrix.
Han, YP; Yan, C; Zhou, L; Qin, L; Tsukamoto, H
The Journal of biological chemistry  282  12928-39  2007

Show Abstract
17322299 17322299
Conditional activation of RET/PTC3 and BRAFV600E in thyroid cells is associated with gene expression profiles that predict a preferential role of BRAF in extracellular matrix remodeling.
Cleo Mesa, Mana Mirza, Norisato Mitsutake, Maureen Sartor, Mario Medvedovic, Craig Tomlinson, Jeffrey A Knauf, Georg F Weber, James A Fagin
Cancer research  66  6521-9  2005

Show Abstract
16818623 16818623
Essential role of matrix metalloproteinases in interleukin-1-induced myofibroblastic activation of hepatic stellate cell in collagen.
Han, YP; Zhou, L; Wang, J; Xiong, S; Garner, WL; French, SW; Tsukamoto, H
The Journal of biological chemistry  279  4820-8  2004

Show Abstract
14617627 14617627

Data Sheet

Title
MOUSE ANTI-RAT MMP-13 (COLLAGENASE-3)