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MABS1703 Anti-MGAT2 Antibody, clone 4A5.2

Detect MGAT2 using this mouse monoclonal Anti-MGAT2 Antibody, clone 4A5.2, Cat. No. MABS1703, validated for use in Western Blotting.

Anti-MGAT2 Antibody, clone 4A5.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: MGAT2, CDG2A UniProt Number: Q10469
MABS1703
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1703
DescriptionAnti-MGAT2 Antibody, clone 4A5.2
Alternate Names
  • Alpha-1
  • 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
  • Beta-1
  • 2-N-acetylglucosaminyltransferase II
  • GlcNAc-T II
  • GNT-II
  • Mannoside acetylglucosaminyltransferase 2
  • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Background InformationAlpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC 2.4.1.143; UniProt Q10469; also known as Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, GNT-II, Mannoside acetylglucosaminyltransferase 2, MGAT2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyl-transferase II) is encoded by the MGAT2 (also known as CDG2A) gene (Gene ID 4247) in human. MGAT2 is a Golgi enzyme that catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans. It possesses the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. It is highly expressed in small intestine in mice and humans. Mutations in MGTA2 gene are known to cause congenital disorders of glycosylation 2A, which is a multi-system disorder caused by defective glycoprotein biosynthesis. It leads to under-glycosylated levels of serum glycoproteins that result in defects in neuronal development, coagulation disorders, hypotonia, and immunodeficiency.

Ref.:
Tan, J., et al. (1995). Eur. J. Biochem. 231, 317-328.
Tan J., et al. (1996), Am. J. Hum. Genet. 159, 810-817.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect MGAT2 using this mouse monoclonal Anti-MGAT2 Antibody, clone 4A5.2, Cat. No. MABS1703, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged internal fragment from the C-terminal half of human MGAT2.
Clone4A5.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4A5.2 targets an internal epitope within the C-terminal half of human MGAT2.
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MGAT2
  • CDG2A
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~52 kDa observed. 51.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected MGAT2 in 10 µg of T47D cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1703 04054839114519