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MABF2044 Anti-MCPyV large T-antigen Antibody, clone CM2B4

Anti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus.

Anti-MCPyV large T-antigen Antibody, clone CM2B4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: N/A
MABF2044
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2044
DescriptionAnti-MCPyV large T-antigen Antibody, clone CM2B4
Alternate Names
  • Large T antigen
  • MCV Large T antigen
Background InformationMerkel cells are neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferents. These cells can turn malignant and form an aggressive form of skin cancer, which is known as Merkel cell carcinoma (MCC). A vast majority of MCC are caused by a polyomavirus known as Merkel cell polyomavirus (MCPyV or MCV). The MCPyV genome is reported to undergo clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Full-length MCPyV large T-antigen is a 125 kDa nuclear protein, however, MCPyV T-antigens obtained from MCC have natural truncating mutations, which result in variably-sized, smaller proteins. Based on homology to other polyomaviruses, the MCPyV large and small T antigens are predicted to be oncogenic and contribute directly to the carcinogenesis of MCC. MCPyV large T antigen can serve as a specific marker for MCC.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MCPyV large T-antigen, clone CM2B4, Cat. No. MABF2044, is a mouse monoclonal antibody that detects MCPyV large T antigen and has been tested for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting for the detection of Merkel cell polyomavirus.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected MCPyV large T-antigen in Western Blotting applications (Liu, X., et. al. (2011). J Biol Chem. 286(19):17079-90; Rodig, S.J., et. al. (2012) J Clin Invest. 122(12):4645-53).

Immunoprecipitation Analysis: A representative lot detected MCPyV large T-antigen in Immunoprecipitation applications (Liu, X., et. al. (2011). J Biol Chem. 286(19):17079-90).

Immunohistochemistry Analysis: A representative lot detected MCPyV large T-antigen in Immunohistochemistry applications (Rodig, S.J., et. al. (2012) J Clin Invest. 122(12):4645-53; Busam, K.J., et. al. (2009). Am J Surg Pathol. 33(9):1378-85).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to RSRKPSSNASRGA sequence from the Merkel cell polyomavirus large T antigen exon 2 with a C-terminal cystiene.
CloneCM2B4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone CM2B4 is highly specific for MCPyV large T and 57kT isoforms, but does not detect MCPyV small T antigen.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • N/A
Purification MethodProtein G purified
Molecular Weight45 kDa observed for truncaed form. For intact form MW 125 kDaUncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MKL-1 cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected MCPyV large T-antigen in 10 µg of MKL-1 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2044 04054839328749

Documentation

Anti-MCPyV large T-antigen Antibody, clone CM2B4 MSDS

Title

Safety Data Sheet (SDS) 

Anti-MCPyV large T-antigen Antibody, clone CM2B4 Certificates of Analysis

TitleLot Number
Anti-MCPyV large T-antigen, clone CM2B4 - 3190907 3190907
Anti-MCPyV large T-antigen, clone CM2B4 - 3689465 3689465
Anti-MCPyV large T-antigen, clone CM2B4 - 3821564 3821564
Anti-MCPyV large T-antigen, clone CM2B4 - 3906513 3906513
Anti-MCPyV large T-antigen, clone CM2B4 - 4028622 4028622
Anti-MCPyV large T-antigen, clone CM2B4 - 4082558 4082558
Anti-MCPyV large T-antigen, clone CM2B4 Monoclonal Antibody Q2906696
Anti-MCPyV large T-antigen, clone CM2B4 Monoclonal Antibody 3090205