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05-503-I Anti-LacI Antibody, clone 9A5

05-503-I
200 µL  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
E. coliWBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-503-I
DescriptionAnti-LacI Antibody, clone 9A5
Alternate Names
  • Lactose operon repressor
Background InformationThe lactose operon repressor binds to a regulatory element, an operator gene, to form a genetic switch, the lac operon. This switch negatively regulates the expression of structural genes that follow the site of the operator gene. Inducers may bind to the repressor to reduce or eliminate the affinity of the repressor for its operator, and thereby allow for transcription of respective structural genes. Conversely, other effector molecules may increase the binding affinity of the repressor for its operator and thereby inhibit gene transcription. The inducer or effector molecules that associate with a repressor serve as a chemical signal, which monitors the metabolic state of protein(s) expressed by their respective genes.
References
Product Information
FormatPurified
Control
  • Rosetta BL21 (DE3) competent E. Coli cell lysate.
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide (This antibody is purified from supernatent.).
Quality LevelMQ100
Applications
ApplicationAnti-LacI Antibody, clone 9A5 is an antibody against LacI for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenTrpE fused with full-length E. Coli LacI.
Clone9A5
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • E. coli
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • lacI
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~38 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in Rosetta™ BL21 (DE3) E. Coli cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected LacI in 10 µg of Rosetta BL21 (DE3) E. Coli cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-503-I 04053252819209