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MABS1219 Anti- LTP/CETP Antibody, clone 3-11D

Anti- LTP/CETP Antibody, clone 3-11D is an antibody against LTP/CETP for use in Western Blotting, ELISA, Inhibits Activity/Function.

Anti- LTP/CETP Antibody, clone 3-11D MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Lipid Metabolism & Weight Regulation Gene Symbol: CETP, HDLCQ10 UniProt Number: P22687
MABS1219
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Rb, MWB, ELISA, INHIBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1219
DescriptionAnti- LTP/CETP Antibody, clone 3-11D
Alternate Names
  • Cholesteryl ester transfer protein
  • CETP
  • Lipid transfer protein I
  • LTP
Background InformationCholesteryl ester transfer protein (UniProt P22687; also known as CETP, Lipid transfer protein I, LTP) is encoded by the CETP (also known as HDLCQ10) gene (Gene ID 1071) in Oryctolagus cuniculus (Rabbit) species. Cholesteryl ester transfer protein (CETP) or lipid transfer protein (LTP) mediates the transport/exhanges of triglycerides and cholesteryl esters (CEs) between very-low-density lipoproteins (VLDL)/low-density lipoproteins (LDL) and high-density lipoproteins (HDL) in both directions. CETP plays an important role in the reverse cholesterol transport (RCT) pathway that mediates the transport of cholesterol from peripheral cells and tissues to the liver for metabolism and excretion in the bile. CETP shuttles CEs from HDL particles to apolipoprotein B-/apoB-containing particles in exchange for triglycerides. CETP is thought to facilitate the generation of small, dense LDL (sd LDL) particles through an indirect mechanism of increased rate of triglyceride transfer from VLDL in exchange for CEs in LDL and HDL. CE transfer between lipoproteins plays a crucial role in intravascular LDL remodeling. Decreased levels of HDL cholesterol and elevated levels of LDL is a risk factor for atherosclerosis and coronary heart disease (CHD).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti- LTP/CETP Antibody, clone 3-11D is an antibody against LTP/CETP for use in Western Blotting, ELISA, Inhibits Activity/Function.
Key Applications
  • Western Blotting
  • ELISA
  • Inhibits Activity/Function
Application NotesELISA Analysis: Representative lots were used as the capture antibody in combination with clone 14–8F as the detection antibody for the detection of human plasma CETP/LTP by sandwich ELISA (Tosheska, K., et al. (2011). Bosn. J. Basic. Med. Sci. 11(3):169–173; Saito, K., et al. J. Lipid Res. (1999). 40(11):2013-2021; Sasai, K., et al. (1998). Clin. Chem. 44(7):1466-1473).
ELISA Analysis: A representative lot was used as the capture antibody in combination with clone 14–8F as the detection antibody for the detection of mouse plasma CETP/LTP by sandwich ELISA (Wu, C.A., et al. (2002). Arterioscler .Thromb. Vasc. Biol. 22(8):1347-1353).
ELISA Analysis: A representative lot showed no significant pH-dependent CETP/LTP-capturing activity in the pH range from 5.0 to 7.5 in sandwich ELISA applications (Saito, K., et al. J. Lipid Res. (1999). 40(11):2013-2021).
Western Blotting Analysis: A representative lot detected only non-reduced, but not reduced, rabbit and human CETP/LTP by Western blotting (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Activity/Function Inhibition Analysis: A representative lot inhibited both rabbit and human CETP/LTP-mediated cholesteryl ester (CE) and triglyceride (TG) transfer from low density lipoprotein (LDL) to high density lipoprotein (HDL) (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Activity/Function Inhibition Analysis: A representative lot blocked the lipid association of both rabbit or human CETP/LTP with microemulsion (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Activity/Function Inhibition Analysis: A representative lot inhibited human CETP/LTP-mediated cholesteryl ester (CE) and triglyceride (TG) transfer from high density lipoprotein (HDL) to to apoB-containing lipoproteins in human plasma (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
Biological Information
ImmunogenNative rabbit plasma CETP/LTP.
Clone3-11D
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3-11D (a.k.a. 3-9F) reacts with non-reduced, but not reduced, LTP/CETP (Ko, K.W., et al. (1994). J. Biol. Chem. 269(45):28206-28213).
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rabbit
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • CETP
  • HDLCQ10
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~70 kDa observed. Target band appears larger than the calculated molecular weight of 54.5 kDa due to glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting of purified human plasma CETP/LTP.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected purified human plasma CETP/LTP under non-reducing condition.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1219 04055977312454