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ABS1634 Anti-LIP5

Detect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting.

Anti-LIP5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: VTA1, C6orf55, HSPC228, My012 UniProt Number: Q9NP79
ABS1634
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABS1634
DescriptionAnti-LIP5
Alternate Names
  • Vacuolar protein sorting-associated protein VTA1 homolog
  • Dopamine-responsive gene 1 protein
  • DRG-1
  • LYST-interacting protein 5
  • LIP5
  • SKD1-binding protein 1
  • SBP1
Background InformationVacuolar protein sorting-associated protein VTA1 homolog (UniProt: Q9NP79; also known as Dopamine-responsive gene 1 protein; DRG-1; LYST-interacting protein 5; LIP5; SKD1-binding protein 1; SBP1) is encoded by the VTA1 (also known as C6orf55; HSPC228; My012) gene (Gene ID 51534) in human. LIP5 is a 33.9 kDa protein involved in trafficking of the multi-vesicular body. It helps regulate the membrane fission and recycling activities of the ESCRT pathway. It performs its central regulatory function within the ESCRT pathway by binding ESCRT-III proteins through its N-terminal tandem microtubule-interacting and trafficking (MIT) domain and by binding VPS4 ATPases through its C-terminal VSL (Vta1/SBP1/LIP5) domain. These interactions allow LIP5 to coordinate ESCRT-III and VPS4 activities both during normal cellular homeostasis and also in response to interferon signaling during innate immune responses. The ESCRT-III and VPS4 ATPase complexes catalyze the membrane fission events associated with these processes. The LIP5 protein helps regulate their interactions by binding directly to a subset of ESCRT-III proteins and to VPS4.
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog), using this rabbit polyclonal Anti-LIP5, Cat. No. ABS1634, validated for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected LIP5 in HeLa cells.

Western Blotting Analysis: A representative lot detected endogenous LIP5 co-immunoprecipiated with Myc-tagged VPS4A from HEK293T cells (Skalicky, J.J., et al. (2012). J Biol Chem. 287(52):43910-43926).
Biological Information
ImmunogenHis-tagged full length human recombinant LIP5.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityDetects LIP5 (Vacuolar protein sorting-associated protein VTA1 homolog) in HeLa cells
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • VTA1
  • C6orf55
  • HSPC228
  • My012
Purification MethodUnpurified.
UniProt Number
Molecular Weight~38 kDa observed. 33.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected LIP5 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1634 04054839135262