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MABT494 Anti-LIMS1, clone 5G7 Antibody

This Anti-LIMS1, clone 5G7 Antibody is validated for use in western blotting, ELISA, flow cytometry & immunofluorescence for the detection of LIMS1.

Anti-LIMS1, clone 5G7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: LIMS1, PINCH, PINCH1 UniProt Number: P48059
MABT494
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ELISA, FC, IFMAscitesMonoclonal Antibody
Description
Catalogue NumberMABT494
DescriptionAnti-LIMS1, clone 5G7 Antibody
Alternate Names
  • LIM and senescent cell antigen-like-containing domain protein 1
  • Particularly interesting new Cys-His protein 1
  • PINCH-1
  • Renal carcinoma antigen NY-REN-48
Background InformationLIM and senescent cell antigen-like-containing domain protein 1 (LIMS1), also known as Particularly interesting new Cys-His protein 1 (PINCH-1; PINCH1; PINCH), or Renal carcinoma antigen NY-REN-48 is an adaptor protein which contains five LIM domains, or double zinc fingers. LIMS1 is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that Lims1 may play a role in integrin-mediated cell adhesion or spreading.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-LIMS1, clone 5G7 Antibody is validated for use in western blotting, ELISA, flow cytometry & immunofluorescence for the detection of LIMS1.
Key Applications
  • Western Blotting
  • ELISA
  • Flow Cytometry
  • Immunofluorescence
Application NotesELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected LIMS1 in HeLa cells.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected LIMS1 in HepG2 cells.
Biological Information
ImmunogenPurified recombinant fragment of human LIMS1 expressed in E. Coli
Clone5G7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • LIMS1
  • PINCH
  • PINCH1
Purification MethodUnpurified
UniProt Number
Molecular Weight~37 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549, Jurkat and HeLa cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected LIMS1 in A549, Jurkat and HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT494 04053252955488