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AB5756 Sigma-AldrichAnti-LIM/Homeobox Protein Lhx2 Antibody

Anti-LIM/Homeobox Protein Lhx2 Antibody is an antibody against LIM/Homeobox Protein Lhx2 for use in IH.

Anti-LIM/Homeobox Protein Lhx2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience, Neuronal & Glial Markers Gene Symbol: LHX2, LH-2, LH2, hLhx2, MGC138390 UniProt Number: P50458

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB5756
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-LIM/Homeobox Protein Lhx2 Antibody
Alternate Names	Homeobox Protein LH-2

References

Product Information
Format	Affinity Purified
Presentation	Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality Level	MQ100

Applications
Application	Anti-LIM/Homeobox Protein Lhx2 Antibody is an antibody against LIM/Homeobox Protein Lhx2 for use in IH.
Key Applications	Immunohistochemistry
Application Notes	Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide from human Lhx2.
Host	Rabbit
Specificity	Recognizes the LIM homeobox containing transcription factor Lhx2.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_004789.3
Entrez Gene Summary	This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution.
Gene Symbol	LHX2 LH-2 LH2 hLhx2 MGC138390
UniProt Number	P50458
UniProt Summary	FUNCTION: SwissProt: P50458 # Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types (By similarity). SIZE: 406 amino acids; 44373 Da SUBCELLULAR LOCATION: Nucleus (Probable). SIMILARITY: SwissProt: P50458 ## Contains 1 homeobox DNA-binding domain. & Contains 2 LIM zinc-binding domains.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
AB5756	04053252271892

Documentation

Anti-LIM/Homeobox Protein Lhx2 Antibody MSDS

Title
Safety Data Sheet (SDS)

Anti-LIM/Homeobox Protein Lhx2 Antibody Certificates of Analysis

Title	Lot Number
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN)	3071601
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) - 2492249	2492249
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) - 3385894	3385894
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) -2584425	2584425
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) -2689080	2689080
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) -2716599	2716599
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) -2769574	2769574
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) AFFINITY PURIFIED POLYCLONAL ANTIBODY	3015852
RABBIT ANTI-LHX2 (LIM/HOMEOBOX PROTEIN) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 3932369	3932369

References

Reference overview	Pub Med ID
LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1. Hou, PS; Chuang, CY; Kao, CF; Chou, SJ; Stone, L; Ho, HN; Chien, CL; Kuo, HC Nucleic acids research 41 7753-70 2013 Show Abstract The LIM homeobox 2 transcription factor Lhx2 is known to control crucial aspects of neural development in various species. However, its function in human neural development is still elusive. Here, we demonstrate that LHX2 plays a critical role in human neural differentiation, using human embryonic stem cells (hESCs) as a model. In hESC-derived neural progenitors (hESC-NPs), LHX2 was found to be expressed before PAX6, and co-expressed with early neural markers. Conditional ectopic expression of LHX2 promoted neural differentiation, whereas disruption of LHX2 expression in hESCs significantly impaired neural differentiation. Furthermore, we have demonstrated that LHX2 regulates neural differentiation at two levels: first, it promotes expression of PAX6 by binding to its active enhancers, and second, it attenuates BMP and WNT signaling by promoting expression of the BMP and WNT antagonist Cerberus 1 gene (CER1), to inhibit non-neural differentiation. These findings indicate that LHX2 regulates the transcription of downstream intrinsic and extrinsic molecules that are essential for early neural differentiation in human.	23804753

Reference overview

Pub Med ID

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