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ST1622 Anti-LEPRE1 Mouse mAb (3C7)

This Anti-LEPRE1 Mouse mAb (3C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of LEPRE1.

Anti-LEPRE1 Mouse mAb (3C7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1

ST1622
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Overview

Replacement Information

Key Spec Table

HostAntibody Type
MMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1622-100UG 100 μg
Description
OverviewRecognizes the ~90 kDa LEPRE1 protein in HeLa cells and human esophagus tissue.
Catalogue NumberST1622
Brand Family Calbiochem®
SynonymsAnti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1
Application Data
Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence.

Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
References
ReferencesChoi, JW, et al. 2009. PLoS Genet. 5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet. 39, 359.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, Human esophagus tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (6 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Clone3C7
HostMouse
IsotypeIgG2a
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1622-100UG 04055977223699

Documentation

Anti-LEPRE1 Mouse mAb (3C7) MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Choi, JW, et al. 2009. PLoS Genet. 5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet. 39, 359.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-November-2010 RFH
SynonymsAnti-Leucine Proline-Enriched Proteoglycan 1, Anti-Leprecan 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (6 µg/ml)
Application Data
Detection of human LEPRE1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human LEPRE1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (10 µg/ml). Detection: fluorescence.

Detection of human LEPRE1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human esophagus tissue. Primary antibody: Anti-LEPRE1 Mouse mAb (3C7) (Cat. No. ST1622) (6 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa LEPRE1 protein.
BackgroundThis gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Two alternatively spliced transcript variants encoding different isoforms have been described. Other variants may exist, but their biological validity has not been determined.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 1-100 of human LEPRE1, expressed as a GST fusion protein
Clone3C7
IsotypeIgG2a
Positive controlHeLa cells, Human esophagus tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesChoi, JW, et al. 2009. PLoS Genet. 5, e1000750.
Cabral, W,A., et al. 2007. Nat Genet. 39, 359.