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AB15504 Anti-Klotho Antibody

Detect Klotho using this Anti-Klotho Antibody validated for use in ELISA & WB.

Anti-Klotho Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: KL, klotho, EC 3.2.1.31 [Contains: Klotho peptide]. UniProt Number: Q9UEF7
AB15504
50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MELISA, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB15504
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Klotho Antibody
Background InformationAnimal models of human aging, which display the characteristic diseases associated with aging, provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span (Kuro-o et al., 1997). KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane (Martin & Mian, 1997). The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial b-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism (Martin & Mian, 1997). kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells (Kuro-o et al., 1997). Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl.
References
Product Information
FormatAffinity Purified
PresentationLiquid in PBS with 0.1% BSA.
Quality LevelMQ100
Applications
ApplicationDetect Klotho using this Anti-Klotho Antibody validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1-10 µg/mL using ECL.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic peptide near the C-terminus of mouse Klotho.
HostRabbit
SpecificityKlotho
Species Reactivity
  • Mouse
Species Reactivity NoteOther species have not yet been tested. The immunogen sequence is 82% conserved in human and 76% in rat klotho.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
Gene Symbol
  • KL
  • klotho
  • EC 3.2.1.31 [Contains: Klotho peptide].
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UEF7 # The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling (By similarity).
SIZE: 1012 amino acids; 116181 Da
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 shedding leads to a soluble peptide. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
DOMAIN:SwissProt: Q9UEF7 Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
PTM: N-glycosylated (By similarity).DISEASE:SwissProt: Q9UEF7 # Defects in KL may be a cause of chronic renal failure complications.
SIMILARITY: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB15504 04053252327292

Documentation

Anti-Klotho Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Klotho Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-KLOTHO 2487600
RABBIT ANTI-KLOTHO 2471886
RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2429664 2429664
RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2437304 2437304
RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2468122 2468122
RABBIT ANTI-KLOTHO - 3222655 3222655
RABBIT ANTI-KLOTHO -2766397 2766397
RABBIT ANTI-KLOTHO AFFINITY PURIFIED 2826996
RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY 2942569
RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY -
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References

Reference overviewPub Med ID
Ageing. New mice for old questions
Martin, G M and Mian, I S
Nature, 390:18-9 (1997)  1997

9363884 9363884
Mutation of the mouse klotho gene leads to a syndrome resembling ageing
Kuro-o, M, et al
Nature, 390:45-51 (1997)  1997

9363890 9363890