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MABE150 Anti-KDM5B Antibody, clone 15G8.1

This Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.

Anti-KDM5B Antibody, clone 15G8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: KDM5B, JARID1B, PLU1, RBBP2H1 UniProt Number: Q9UGL1
MABE150
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE150
DescriptionAnti-KDM5B Antibody, clone 15G8.1
Alternate Names
  • Lysine-specific demethylase 5B
  • KDM5B
  • Cancer/testis antigen 31
  • CT31
  • Histone demethylase JARID1B
  • Jumonji/ARID domain-containing protein 1B
  • PLU-1
  • Retinoblastoma-binding protein 2 homolog 1
  • RBP2-H1
Background InformationLysine-specific demethylase 5B (UniProt Q9UGL1; also known as Cancer/testis antigen 31, CT31, Histone demethylase JARID1B, Jumonji AT rich interactive domain 1B, Jumonji/ARID domain-containing protein 1B, PLU-1, Protein phosphatase 1 regulatory subunit 98, Putative DNA/chromatin binding motif, RBP2-H1, Retinoblastoma-binding protein 2 homolog 1) is encoded by the KDM5B (also known as PLU1, PUT1, JARID1B, PPP1R98, RBBP2H1, RBBP2H1A) gene (Gene ID 10765) in human. JARID1B/KDM5B is an H3K4me2/3 demethylase that regulates self-renewal and differentiation in a cell type-specific manner. JARID1B/KDM5B is highly expressed in embryonic stem cells (ESCs), hematopoietic stem cells (HSCs), and in various primary cancers including melanoma, breast, testicular, and is upregulated in leukemic cell lines.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-KDM5B Antibody, clone 15G8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of KDM5B.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected KDM5B in 10 µg of MCF-7 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected KDM5B in human kidney and mouse cerebral cortex tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the region between ARID and JmjC domain of human KDM5B.
EpitopeRegion between ARID and JmjC domain
Clone15G8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KDM5B
  • JARID1B
  • PLU1
  • RBBP2H1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~175 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected KDM5B in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE150 04055977288353

Documentation

Anti-KDM5B Antibody, clone 15G8.1 MSDS

Title

Safety Data Sheet (SDS) 

Anti-KDM5B Antibody, clone 15G8.1 Certificates of Analysis

TitleLot Number
Anti-KDM5B, clone 15G8.1 3170177
Anti-KDM5B, clone 15G8.1 - 3070186 3070186
Anti-KDM5B, clone 15G8.1 - 3386489 3386489
Anti-KDM5B, clone 15G8.1 - 4082591 4082591
Anti-KDM5B, clone 15G8.1 -Q2608335 Q2608335
Anti-KDM5B, clone 15G8.1 Monoclonal Antibody 2907162

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications