Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
			96-Well Plate

Qty	Catalogue Number	Ordering Description	Qty/Pack	List Price
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MAB1976-AF555 Sigma-AldrichAnti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate is an antibody against Integrin αVβ3 for use in Immunocytochemistry.

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
Brochures
Posters

Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: ITGAV, MSK8, VNRA UniProt Number: P06756

Recommended Products

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
Av, Rb, B, Ca, Ch, H, Mk, Po	ICC	M	AlexaFluor®555	Monoclonal Antibody

Description
Catalogue Number	MAB1976-AF555
Description	Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate
Alternate Names	Integrin alpha-V Vitronectin receptor subunit alpha CD51 Integrin alpha-V heavy chain Integrin alpha-V light chain Integrin αVβ3
Background Information	Integrin alpha-V (CD51, Vitronectin receptor subunit alpha; UniProt P06756) and Integrin beta-3 (CD61, GPIIIa, Platelet membrane glycoprotein IIIa; UniProt P05106) are encoded by the ITGAV (MSK8, VNRA; Gene ID 3685) and ITGB3 (GP3A; Gene ID 3690) gene, respectively, in human. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and a beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue. Due to its involvement in angiogenesis, integrin alphaVbeta3 is among of the most studied integrin receptors.

References

Product Information
Format	AlexaFluor®555
Presentation	Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate is an antibody against Integrin αVβ3 for use in Immunocytochemistry.
Key Applications	Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Integrin αVβ3 in HeLa cells. The unconjugated antibody (Cat. No. MAB1976) is shown to be suitable also for functional assay, flow cytometry, immunofluorescence, immunohistochemistry, and immunoprecipitation applications.

Biological Information
Immunogen	Purified adhesion receptor from the human melanoma cell line M21 (Cheresh, D.A., and Spiro R.C. (1987). J. Biol. Chem. 262(36):17703-11).
Clone	LM609
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Specificity	Reacts with the vitronectin receptor alphaVbeta3 complex, an RGD-directed adhesion receptor. LM609 has been demonstrated to block adhesion of a human melanoma cell line (M21) to vitronectin, fibrinogen and von Willebrand factor, as well as to a synthetic RGD containing peptide (Cheresh, D.A., and Spiro R.C. (1987). J Biol Chem. 262(36):17703-11; Cheresh, D.A. (1987). Proc. Natl. Acad. Sci. U.S.A. 84(18):6471-6475). In chick chorioallentoic membranes, LM609 was shown to block angiogenesis induced by bFGF and TNFalpha but had no effect on pre-existing vessels (Brooks, P.C., et al. (1994). Science. 264(5158):569-571). While LM609 does block cell attachment to RGD containing ligands, it does not interact directly with the RGD binding site. Instead, LM609 appears to be an allosteric inhibitor of integrin alphaVbeta3, which binds to a conformational epitope resulting from the post-translational association of the alphaV and beta3 subunits.
Isotype	IgG1
Species Reactivity	Avian Rabbit Bovine Canine Chicken Human Monkey Pig
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_002210.2
Gene Symbol	ITGAV MSK8 VNRA
Purification Method	Protein A Purfied
UniProt Number	P06756
Molecular Weight	~116 kDa observed

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in HepG2 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin αVβ3 in HepG2 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MAB1976-AF555	04055977294729

Documentation

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate MSDS

Title
Safety Data Sheet (SDS)

Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor® 555 Conjugate Certificates of Analysis

Title	Lot Number
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate	2907161
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate	3792043
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3230943	3230943
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3722057	3722057
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate - 3845817	3845817
Anti-Integrin αVβ3, clone LM609, Alexa Fluor® 555 Conjugate -Q2611359	Q2611359

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications

Related Products & Applications

Related Products

Catalogue Number	Description
AB1930	Anti-Integrin alphaV Antibody, CT, Intracellular
CBL490	Anti-Integrin αV Antibody, clone 13C2
FCMAB282F	Milli-Mark™ Anti-Integrin αVβ3 Antibody, clone LM609-FITC
MAB1876	Anti-Integrin αVβ3 Antibody, clone 27.1 (VNR-1)
MAB1953	Anti-Integrin αV Antibody, clone P3G8
MAB1953Z	Anti-Integrin αV Antibody, clone P3G8, azide free
MAB1961	Anti-Integrin αVβ5 Antibody, clone P1F6
MAB1961F	Anti-Integrin αVβ5 Antibody, clone P1F6, FITC conjugated
MAB1961Z	Anti-Integrin αVβ5 Antibody, clone P1F6, azide free
MAB1976	Anti-Integrin αVβ3 Antibody, clone LM609

The Product Has Been Added To Your Cart