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CP26 Anti-Integrin β₁ Mouse mAb (4B7)

Anti-Integrin β₁ Mouse mAb (4B7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP26
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the β1 integrin subunit in A431 and Jurkat cells.
Catalogue NumberCP26
Brand Family Calbiochem®
References
ReferencesAkiyama, S.K. 1996. Hum. Cell. 9, 181.
Farnoud, M.R. et al. 1996. Int. J. Cancer 67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell. 9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem. 61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol. 130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem. 269, 15961.
Albelda, S.M. 1993. Lab. Invest. 68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep. 16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Positive controlA-431 or Jurkat cells or skin tissue
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesNeutralization Studies Heise, T. and Dersch, P. 2006. Proc. Natl. Acad. Sci. USA 103, 3375.
Key Applications Frozen Sections
Immunofluorescence
Immunoprecipitation
Not Western Blot
Neutralization Studies
Paraffin Sections
Application NotesFrozen Sections (2 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2 µg/ml, no pre-treatment required)
Immunoblotting (not recommended)
Neutralization Studies (see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human β1 integrin
ImmunogenHuman
Clone4B7
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CP26 0

Documentation

Anti-Integrin β₁ Mouse mAb (4B7) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin β₁ Mouse mAb (4B7) Certificates of Analysis

TitleLot Number
CP26

References

Reference overview
Akiyama, S.K. 1996. Hum. Cell. 9, 181.
Farnoud, M.R. et al. 1996. Int. J. Cancer 67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell. 9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem. 61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol. 130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem. 269, 15961.
Albelda, S.M. 1993. Lab. Invest. 68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep. 16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-March-2008 JSW
ApplicationFrozen Sections (2 µg/ml)
Immunofluorescence (2.5 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (2 µg/ml, no pre-treatment required)
Immunoblotting (not recommended)
Neutralization Studies (see application references)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the β1 integrin subunit.
BackgroundThe integrins are a family of more than 23 heterodimeric transmembrane proteins that mediate cell-cell adhesions as well as cell-substratum adhesions and signal transduction processes. Expressed on essentially all cells as an alpha-beta dimeric protein, alterations in the integrin structure leading to changes in adhesive properties have important implications for tumor invasiveness and progression. The majority of the protein is found in the globular extracellular domain with the intracellular domain only composed of 60 amino acids or less. The beta 1 integrin is found widely distributed and is one of the subunits composing the major fibronectin receptor (FNR) along with the alpha 5 integrin. The beta 1 integrin has been shown to have a direct as well as a regulatory role in ligand binding and subsequent signal transduction. Upon ligand binding there is initiated a signal transduction cascade involving the phosphorylation of the integrin molecule itself as well as any of a number of different substrates including pp125FAK, PLCγ, MAP kinase and others. In addition to the role played in normal cell-cell interactions and development, integrins play an important role in the etiology of cancer.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human β1 integrin
Clone4B7
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlA-431 or Jurkat cells or skin tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Protect from moisture
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesAkiyama, S.K. 1996. Hum. Cell. 9, 181.
Farnoud, M.R. et al. 1996. Int. J. Cancer 67, 45.
Morimoto, C. and Tachibana, K. 1996. Hum. Cell. 9, 163.
Webb, D.L. et al. 1996. J. Cell. Biochem. 61, 127.
Schaller, M.D. et al. 1995. J. Cell. Biol. 130, 1181.
Akiyama, S.K. et al. 1994. J. Biol. Chem. 269, 15961.
Albelda, S.M. 1993. Lab. Invest. 68, 4.
Evans, C.W. 1992. Cell. Biol. Int. Rep. 16, 1.
Albelds, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
Application referencesNeutralization Studies Heise, T. and Dersch, P. 2006. Proc. Natl. Acad. Sci. USA 103, 3375.