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MAB19294 Anti-Integrin β1 Antibody, active β1 integrins, clone TASC/9D11

MAB19294
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
ChIP, ICC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB19294
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Integrin β1 Antibody, active β1 integrins, clone TASC/9D11
Alternate Names
  • CD29
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in 0.02M PB +0.25 M NaCl, pH=7.6 with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationDetect the Integrin β1 protein using this Anti-Integrin β1, active β1 integrins, clone TASC/9D11 validated for use in IP & ICC.
Key Applications
  • Immunoprecipitation
  • Immunocytochemistry
  • Affects Function
Application NotesWestern blot: Not effective

Immunocytochemistry

Immunoprecipitation

Biological activity: Functionally inhibits attachment to vitronectin. Activates attachment to other substrates.



Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenE10 chick brain glial cells
Epitopeactive beta1 integrins
CloneTASC/9D11
HostMouse
SpecificityReactive with the extracellular domain of chicken integrin beta 1
IsotypeIgG1
Species Reactivity
  • Chicken
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
Gene Symbol
  • ITGB1
  • GPIIA
  • OTTHUMP00000063731
  • OTTHUMP00000046253
  • OTTHUMP00000063733
  • VLAB
  • OTTHUMP00000063732
  • MDF2
  • FNRB
  • CD29
  • MSK12
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05556 # Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta- 1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha- 1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha- 3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha- 10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform beta-1B interferes with isoform beta-1A resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.


SIZE: 798 amino acids; 88465 Da

SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-1 associates with either alpha-1, alpha-2, alpha-3, alpha-4, alpha- 5, alpha-6, alpha-7, alpha-8, alpha-9, alpha-10, alpha-11 or alpha-V. Interacts with FLNA. Binds LGALS3BP and ITGB1BP3, when associated with alpha-7, but not with alpha-5 (By similarity). Interacts with FLNB and RANBP9. Isoform Beta-1D interacts with ACE2. Isoform Beta-1A interacts with the C-terminal region of FLNC. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Isoform beta-1B does not localize to focal adhesions. Highly enriched in stage I melanosomes.

TISSUE SPECIFICITY: Isoform beta-1A is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform beta-1B is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbelical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform beta-1C and isoform beta-1C-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-C-2, rather than isoform beta-1C, is selectively expressed in primary T-cells. Isoform beta-1C is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1D is expressed specifically in striated muscle (skeletal and cardiac muscle).





SIMILARITY: SwissProt: P05556 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB19294 04053252319006

Documentation

Anti-Integrin β1 Antibody, active β1 integrins, clone TASC/9D11 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin β1 Antibody, active β1 integrins, clone TASC/9D11 Certificates of Analysis

TitleLot Number
MOUSE ANTI-CHICKEN INTEGRIN BETA1 2477856
MOUSE ANTI-CHICKEN INTEGRIN BETA1 MONOCLONAL ANTIBODY - 2437308 2437308
MOUSE ANTI-CHICKEN INTEGRIN BETA1 - 3471985 3471985
MOUSE ANTI-CHICKEN INTEGRIN BETA1 - 3615484 3615484
MOUSE ANTI-CHICKEN INTEGRIN BETA1 - 3731753 3731753
MOUSE ANTI-CHICKEN INTEGRIN BETA1 - 3897687 3897687
MOUSE ANTI-CHICKEN INTEGRIN BETA1 -2530108 2530108
MOUSE ANTI-CHICKEN INTEGRIN BETA1 -2549989 2549989
MOUSE ANTI-CHICKEN INTEGRIN BETA1 -2569162 2569162
MOUSE ANTI-CHICKEN INTEGRIN BETA1 -2649173 2649173

References

Reference overviewPub Med ID
Integrins and cAMP mediate netrin-induced growth cone collapse.
Lemons, ML; Abanto, ML; Dambrouskas, N; Clements, CC; Deloughery, Z; Garozzo, J; Condic, ML
Brain research  1537  46-58  2013

Show Abstract
24001590 24001590
Neural crest ontogeny during secondary neurulation: a gene expression pattern study in the chick embryo.
Osório, L; Teillet, MA; Palmeirim, I; Catala, M
The International journal of developmental biology  53  641-8  2009

Show Abstract
19247972 19247972
Combined integrin activation and intracellular cAMP cause Rho GTPase dependent growth cone collapse on laminin-1.
M L Lemons, M L Condic
Experimental neurology  202  324-35  2005

Show Abstract
16899244 16899244
Vitronectin and thrombospondin promote retinal neurite outgrowth: developmental regulation and role of integrins.
Neugebauer, K M, et al.
Neuron, 6: 345-58 (1991)  1991

Show Abstract
1705807 1705807
Cell-surface regulation of beta 1-integrin activity on developing retinal neurons.
Neugebauer, K M and Reichardt, L F
Nature, 350: 68-71 (1991)  1991

Show Abstract
1706071 1706071