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GR36 Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3)

This Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) is validated for use in Frozen Sections, Immunoblotting, IP, Paraffin Sections for the detection of Insulin Receptor (β-Subunit).

Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
GR36
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
OverviewRecognizes the ~95 kDa the β-subunit of insulin receptor in IM9 cells, placenta, and fetal liver tissue.
Catalogue NumberGR36
Brand Family Calbiochem®
Application Data
Detection of human insulin receptor (β-subunit) by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) (Cat. No. GR36) (1:50). Detection: fluorescence.

Detection of human insulin receptor (β-subunit) by immunoblotting. Sample: Human insulin receptor. Primary antibody: Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) (Cat. No. GR36) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesGrigorescu, F., et al. 1987. J.Clin. Endocrinol. Metab. 64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Rosen, O.M. 1987. Science 237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ganguly, S., et al. 1985. In Current Topics in Cellular Regulation, Academic Press 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlIM-9 lymphocyte cells or placenta or liver tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Not Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunoprecipitation (not recommended)
Paraffin Sections (1:50-1:100, pressure cooker pre-treatment required)
Application CommentsNo cross-reactivity with IGF receptors. Will stain IM-9 lymphocyte cells as well as placenta and liver tissue. This antibody is also useful for tyrosine kinase assays for antibody mediated capture on microwell plates. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of the C-terminal 100 amino acids of the β-subunit of human insulin receptor fused to GST
ImmunogenHuman
CloneCT-3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles. Centrifuge the contents of the vial containing the antibody before use to remove any solution that may have accumulated around or under the cap of the vial during shipment or storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR36 0

Documentation

Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) Certificates of Analysis

TitleLot Number
GR36

References

Reference overview
Grigorescu, F., et al. 1987. J.Clin. Endocrinol. Metab. 64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Rosen, O.M. 1987. Science 237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ganguly, S., et al. 1985. In Current Topics in Cellular Regulation, Academic Press 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
ApplicationFrozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunoprecipitation (not recommended)
Paraffin Sections (1:50-1:100, pressure cooker pre-treatment required)
Application Data
Detection of human insulin receptor (β-subunit) by immunohistochemistry. Sample: Human placenta tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) (Cat. No. GR36) (1:50). Detection: fluorescence.

Detection of human insulin receptor (β-subunit) by immunoblotting. Sample: Human insulin receptor. Primary antibody: Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) (Cat. No. GR36) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody gernerated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~95 kDa the β-subunit of insulin receptor.
BackgroundInsulin initiates its effects on cells through interaction with high-affinity, cell surface, glycoprotein receptors, consisting of two α (~135 kDa) and two β (~95 kDa) subunits linked by disulfide bonds. The α subunits, which are completely extracellular, bind insulin and the β subunits possessing a single transmembrane domain become phosphorylated in response to insulin. Insulin-dependent phosphorylation occurs at multiple sites near the carboxyl terminus and in the kinase domain. The receptor protein-tyrosine kinase has been implicated as the mediator of most, if not all, of the effects of insulin. Mutations that diminish both phosphorylation and internalization of the insulin receptor block insulin action, as do antibodies, which inhibit its protein-tyrosine kinase activity. Cells from patients with diabetic syndromes, characterized by non-responsiveness to insulin, exhibit reduced insulin dependent protein-tyrosine kinase activity.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of the C-terminal 100 amino acids of the β-subunit of human insulin receptor fused to GST
CloneCT-3
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlIM-9 lymphocyte cells or placenta or liver tissue
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsNo cross-reactivity with IGF receptors. Will stain IM-9 lymphocyte cells as well as placenta and liver tissue. This antibody is also useful for tyrosine kinase assays for antibody mediated capture on microwell plates. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles. Centrifuge the contents of the vial containing the antibody before use to remove any solution that may have accumulated around or under the cap of the vial during shipment or storage.
Toxicity Standard Handling
ReferencesGrigorescu, F., et al. 1987. J.Clin. Endocrinol. Metab. 64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem. 262, 15341.
Rosen, O.M. 1987. Science 237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 328.
Morgan, D.O. and Roth, R.A. 1986. Biochemistry 25, 1364.
White, M.F. and Kahn, C.R. 1986. The Enzymes 17, 247.
Ganguly, S., et al. 1985. In Current Topics in Cellular Regulation, Academic Press 27, 83.
Grunberger, G., et al. 1984. Science 223, 932.