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MABF2754B-100UG Anti-IL1F10 (IL-38) Antibody, clone H127C Antibody, biotin conjugate

Anti-IL1F10 (IL-38), clone H127C, biotin conjugate, Cat. No. MABF2754B, is a mouse monoclonal antibody that detects Interleukin-1 family member 10 (IL1F10) and is tested for use in ELISA.

MABF2754B-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2754B-100UG
DescriptionAnti-IL1F10 (IL-38) Antibody, clone H127C Antibody, biotin conjugate
Alternate Names
  • Interleukin-1 family member 10
  • 1F10
  • Family of interleukin 1-theta
  • FIL1 theta
  • Interleukin-1 HY2
  • IL-1HY2
  • IL-1 theta
  • Interleukin-38
  • IL-38
Background InformationInterleukin-1 family member 10 (UniProt: Q8WWZ1; also known as IL-1F10, Family of interleukin 1-theta, FIL1 theta, Interleukin-1 HY2, IL-1HY2, Interleukin-1 theta, IL-1 theta, Interleukin-38, IL-38) is encoded by the IL1F10 (also known as FIL1T, IL1HY2, IL38, FKSG75, UNQ6119/PRO20041) gene (Gene ID: 84639) in human. IL-38 is a cytokine with immunomodulatory properties. It is reported to be expressed in fetal skin, spleen and tonsil. Its expression is mostly in the basal epithelia of skin and in proliferating B-cells of the tonsil. It is also shown to be strongly expressed in synovia of rheumatoid arthritis (RA) subjects and is reported to exert anti-inflammatory effects in a murine model of RA. IL-38 alone does not induce cytokine production, but reduces IL-22 and IL-17A production by T cells in response to heat-killed Candida albicans. It also increases the production of IL-6 by dendritic cells stimulated with lipopopolysaccharides. This biotinylated antibody is suitable as a detection antibody for IL-38. (Ref.: Takenaka, S., et al. (2015). Biochem. Biophys. Rep. 4; 386-391; van de Veerdonk, FL., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(8); 3001-3005).
References
Product Information
FormatBiotin
PresentationPurified mouse monoclonal antibody IgG2b in PBS, 1.5% BSA with 0.1% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-IL1F10 (IL-38), clone H127C, biotin conjugate, Cat. No. MABF2754B, is a mouse monoclonal antibody that detects Interleukin-1 family member 10 (IL1F10) and is tested for use in ELISA.
Key Applications
  • ELISA
Application NotesELISA Analysis: A representative lot detected IL1F10 (IL-38) in ELISA applications (Takenaka, S.I., et. al. (2015). Biochem Biophys Rep. 4:386-391).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length recombinant human Interleukin-1 family member 10 (IL1F10).
EpitopeUnknown
CloneH127C
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone H127C is a mouse monoclonal antibody that detects human IL11F10 (IL-38). This biotinylated antibody is suitable for use as a detection antibody.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL1F10
  • FIL1T
  • IL1HY2
  • IL38
  • FKSG75
  • UNQ6119/PRO20041
Purification MethodProtein G purified
UniProt Number
Molecular Weight16.94 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with recombinant human IL1F10 (IL-38).

ELISA Analysis: Titration, starting at 600 ng/mL, two-fold serial dilutions (11 pts) of this antibody detected recombinant human IL1F10 (IL-38).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2754B-100UG 04061842625970

Documentation

Anti-IL1F10 (IL-38) Antibody, clone H127C Antibody, biotin conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-IL1F10 (IL-38) Antibody, clone H127C Antibody, biotin conjugate Certificates of Analysis

TitleLot Number
Anti-IL1F10 (IL-38), clone H127C, biotin conjugate - Q3503102 Q3503102

References

Reference overviewPub Med ID
IL-38: A new factor in rheumatoid arthritis
Shin-Ichi Takenaka 1 , Shinjiro Kaieda 1 , Tomotaka Kawayama 1 , Masanobu Matsuoka 1 , Yoichiro Kaku 1 , Takashi Kinoshita 1 , Yuki Sakazaki 1 , Masaki Okamoto 1 , Masaki Tominaga 1 , Katsuya Kanesaki 2 , Asako Chiba 3 , Sachiko Miyake 3 , Hiroaki Ida 1 , Tomoaki Hoshino
Biochem Biophys Rep  4  386-391  2015

Show Abstract 		29124228 29124228