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OP134 Anti-IKKβ Mouse mAb (10AG2)

This Anti-IKKβ Mouse mAb (10AG2) is validated for use in Immunoblotting for the detection of IKKβ.

Anti-IKKβ Mouse mAb (10AG2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP134
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~87 kDa IKKβ protein in Daudi cells.
Catalogue NumberOP134
Brand Family Calbiochem®
Application Data
Detection of human IKKβ by immunoblotting. Sample: Whole cell lysate from Daudi cells (30 µg). Primary antibody: Anti-IKKβ Mouse mAb (10AG2) (Cat. No. OP134) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesDelhase, M., et al. 1999. Science 284, 309.
Li. Q., et al. 1999. Science 284, 321.
Didonato, et al. 1997. Nature 388, 548.
Lee, F.S., et al. 1997. Cell 88, 213.
Malinin, N.L., et al. 1997. Nature 385, 540.
Mercurio, F., et al. 1997. Science 278, 860.
Regnier, C.H., et al. 1997. Cell 90, 373.
Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA 94, 11758.
Verma, I.M., et al. 1995. Genes. Dev. 9, 2723.
Product Information
FormLiquid
FormulationIn PBS, 0.2% gelatin, pH 7.4.
Positive controlDaudi cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-4 µg/ml, chemiluminescence)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of full-length human IKKβ fused to a His•Tag® sequence
ImmunogenHuman
Clone10AG2
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP134 0

Documentation

Anti-IKKβ Mouse mAb (10AG2) MSDS

Title

Safety Data Sheet (SDS) 

Anti-IKKβ Mouse mAb (10AG2) Certificates of Analysis

TitleLot Number
OP134

References

Reference overview
Delhase, M., et al. 1999. Science 284, 309.
Li. Q., et al. 1999. Science 284, 321.
Didonato, et al. 1997. Nature 388, 548.
Lee, F.S., et al. 1997. Cell 88, 213.
Malinin, N.L., et al. 1997. Nature 385, 540.
Mercurio, F., et al. 1997. Science 278, 860.
Regnier, C.H., et al. 1997. Cell 90, 373.
Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA 94, 11758.
Verma, I.M., et al. 1995. Genes. Dev. 9, 2723.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationImmunoblotting (1-4 µg/ml, chemiluminescence)
Application Data
Detection of human IKKβ by immunoblotting. Sample: Whole cell lysate from Daudi cells (30 µg). Primary antibody: Anti-IKKβ Mouse mAb (10AG2) (Cat. No. OP134) (1 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~87 kDa IKKβ protein.
BackgroundNF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/γ. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKK2/β not IKK1/α, is the target proinflammatory stimuli. Targeted disruption of IKK2/β gene in mice also results in extensive liver damage from apotopsis and death as embryo.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of full-length human IKKβ fused to a His•Tag® sequence
Clone10AG2
IsotypeIgG₁
Specieshuman
Positive controlDaudi cells
FormLiquid
FormulationIn PBS, 0.2% gelatin, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDelhase, M., et al. 1999. Science 284, 309.
Li. Q., et al. 1999. Science 284, 321.
Didonato, et al. 1997. Nature 388, 548.
Lee, F.S., et al. 1997. Cell 88, 213.
Malinin, N.L., et al. 1997. Nature 385, 540.
Mercurio, F., et al. 1997. Science 278, 860.
Regnier, C.H., et al. 1997. Cell 90, 373.
Verma, I. And Stevenson, J.K. 1997. Proc. Nat. Acad. Sci. USA 94, 11758.
Verma, I.M., et al. 1995. Genes. Dev. 9, 2723.