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MABS1301 Anti-IGF-IRα subunit Antibody, clone 1B1

This Anti-IGF-IRα subunit Antibody, clone 1B1 is validated for use in Flow Cytometry, Immunocytochemistry, Western Blotting for the detection of IGF-IRα subunit.

Anti-IGF-IRα subunit Antibody, clone 1B1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: IGF1R, CD221, IGFIR, IGFR, JTK13 UniProt Number: P08069
MABS1301
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1301
DescriptionAnti-IGF-IRα subunit Antibody, clone 1B1
Alternate Names
  • Insulin-like growth factor 1 receptor
  • CD221
  • IGF-I receptor
  • Insulin-like growth factor I receptor
  • Insulin-like growth factor 1 receptor alpha chain
  • Insulin-like growth factor 1 receptor beta chain
  • IGF-IRα subunit
Background InformationInsulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGF-1 receptor is initially produced as a precursor protein with a signal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-IGF-IRα subunit Antibody, clone 1B1 is validated for use in Flow Cytometry, Immunocytochemistry, Western Blotting for the detection of IGF-IRα subunit.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Western Blotting
Application NotesFlow Cytometry Analysis: A representative lot detected IGF-I receptor on the surface of MCF-7 human breast cancer cells (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128).
Immunocytochemistry Analysis: A representative lot detected IGF-I receptor on the surface of MCF-7 human breast cancer cells (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128).
Western Blotting Analysis: A representative lot detected the extracellular alpha (aa 31-736) chain of IGF-1R in MCF-7 cell extracts (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128).
Biological Information
ImmunogenRecombinant protein corresponding to the extracellular fragment of human IGF-IR precursor protein.
EpitopeIGF-IR extracellular alpha chain.
Clone1B1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1B1 detected a band corresponding to the size of the alpha subunit of IGF-1R by immunoblotting analysis of recombinant protein and MCF-7 cell extracts (Nakadate, Y., et al. (2014). Monoclon. Antib. Immunodiagn. Immunother. 33(2):126-128).
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • IGF1R
  • CD221
  • IGFIR
  • IGFR
  • JTK13
Purification MethodProtein G Purified
UniProt Number
Molecular Weight80.41 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in MCF7 cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected IGF-IRα subunit on MCF7 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1301 04055977314991

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies