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GR11 Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of IGF-IR (Ab-1).

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-IGF-R, Anti-Insulin-Like Growth Factor Receptor

GR11
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
GR11-100UG Plastová ampulka 100 μg
Description
OverviewRecognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
Catalogue NumberGR11
Brand Family Calbiochem®
SynonymsAnti-IGF-R, Anti-Insulin-Like Growth Factor Receptor
References
ReferencesRoth, R. 1988. Science 239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
Rosen, O.M., 1987. Science 257, 1452.
Rechler, M.M. and Nissley, S.P., 1986. Hormone Res. 24, 152.
Ullrich, A., et al. 1986. EMBO J. 5, 2503.
Zapf, S. and Froesch, E.R., 1986. Hormone Res. 24, 121.
Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).
Kull, F.C., et al. 1983. J. Biol. Chem. 258, 6561.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlHS27 cells
Positive controlHepG2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunofluorescence
Immunoprecipitation
Not Western Blot
Not Paraffin Sections
Application NotesImmunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Neutralization Studies (1 µg/ml; use Cat. No. GR11L)
Paraffin Sections (not recommended)
Application CommentsAnti-IGF-I Receptor (Ab-1) Mouse mAb (αIR3) immunoprecipitates the α and β subunits of the IGF-I receptor. Blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in indvidual systems.
Biological Information
Immunogenpartially purified, human placenta IGF-I receptor
ImmunogenHuman
CloneαIR3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GR11-100UG 04055977228229

Documentation

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) MSDS

Title

Safety Data Sheet (SDS) 

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) Certificates of Analysis

TitleLot Number
GR11

References

Reference overview
Roth, R. 1988. Science 239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
Rosen, O.M., 1987. Science 257, 1452.
Rechler, M.M. and Nissley, S.P., 1986. Hormone Res. 24, 152.
Ullrich, A., et al. 1986. EMBO J. 5, 2503.
Zapf, S. and Froesch, E.R., 1986. Hormone Res. 24, 121.
Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).
Kull, F.C., et al. 1983. J. Biol. Chem. 258, 6561.

Citations

Title
  • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
  • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
  • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision24-August-2007 RFH
    SynonymsAnti-IGF-R, Anti-Insulin-Like Growth Factor Receptor
    ApplicationImmunoblotting (not recommended)
    Immunofluorescence (1-5 µg/ml)
    Immunoprecipitation (1-2 µg/sample)
    Neutralization Studies (1 µg/ml; use Cat. No. GR11L)
    Paraffin Sections (not recommended)
    DescriptionPurified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.
    BackgroundThe insulin-like growth factors (IGFs) or somatomedins are mitogenic peptides present in the plasma of human, rat, and other species. Two distinct but chemically related IGFs have been purified from human plasma: IGF-I (Somatomedin C) and IGF-II. They are single chain polypeptides with molecular weights of 7,640 and 7,471, respectively. Types I and II IGF receptors differ in their structure and in their peptide binding specificity. Type I IGF receptors are structurally homologous to insulin receptors and contain α-subunits (Mr=130,000) that bind peptide, and β-subunits (Mr=90,000), which have intrinsic tyrosine kinase activity. The protein-tyrosine kinase activity of the insulin receptor and, presumably the IGF-I receptor, is required for insulin to stimulate all of its biological responses in cells. Type II receptors are a single polypeptide (Mr=260,000), lack protein-tyrosine kinase activity, and have been shown to be identical to the mannose-6-phosphate receptor.
    HostMouse
    Immunogen speciesHuman
    Immunogenpartially purified, human placenta IGF-I receptor
    CloneαIR3
    IsotypeIgG₁
    Specieshuman
    Positive controlHepG2 cells
    Negative controlHS27 cells
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAnti-IGF-I Receptor (Ab-1) Mouse mAb (αIR3) immunoprecipitates the α and β subunits of the IGF-I receptor. Blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in indvidual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesRoth, R. 1988. Science 239, 1269.
    Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm. 149, 276.
    Rosen, O.M., 1987. Science 257, 1452.
    Rechler, M.M. and Nissley, S.P., 1986. Hormone Res. 24, 152.
    Ullrich, A., et al. 1986. EMBO J. 5, 2503.
    Zapf, S. and Froesch, E.R., 1986. Hormone Res. 24, 121.
    Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).
    Kull, F.C., et al. 1983. J. Biol. Chem. 258, 6561.
    Citation
  • Rita Nahta, et al. (2005) Insulin-like growth factor-I receptor/human epidermal growth factor receptor 2 heterodimerization contributes to trastuzumab resistance of breast cancer cells. Cancer Research 65, 11118-11128.
  • Daniel E. Syroid, et al. (1999) A role for insulin-like growth factor-I in the regulation of Schwann cell survival. Journal of Neuroscience 19, 2059-2068.
  • Donald M. Henricks, et al. (1998) Identification of insulin-like growth factor I in bovine seminal plasma and its receptor on spermatozoa: influence on sperm motility. Biology of Reproduction 59, 330-337.
    		•