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ABE154 Anti-Histone H3.1/H3.2 Antibody

Anti-Histone H3.1/H3.2 Antibody is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting & ChIP-seq.

Anti-Histone H3.1/H3.2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ UniProt Number: P68431
ABE154
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ChIP-seq, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE154
DescriptionAnti-Histone H3.1/H3.2 Antibody
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/I
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
Background InformationHistone H3.1 and H3.2 are closely related histone variants that differ by only one amino acid. Mammalian cells express three major types of noncentromeric histone H3 variants, H3.1, H3.2, and H3.3. H3.1 is only expressed in mammals, while H3.2 is found in all eukaryotes except for yeast. Recent evidence shows that these nearly identical histone proteins differ in their expression profiles and post-translational modifications (PTMs). H3.2 is enriched in modifications associated with transcriptional silencing (di- and tri-methylation of K27), whereas H3.1 is enriched in both K9 dimethylation and K14 acetylation, suggesting that these variants may have separate biological functions.
References
Product Information
FormatAffinity Purified
Control
  • HeLa acid extract
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Histone H3.1/H3.2 Antibody is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting & ChIP-seq.
Key Applications
  • Western Blotting
  • ChIP-seq
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blotting Analysis: A representative lot detected Histone H3.2 in recombinant Histone H3.2 and mouse Embryonic Stem cell lysates.

Chromatin Immunprecipitation: A representative lot detected Histone H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).

Chromatin Immunoprecipitation Sequencing: A representative lot detected Histone H3.1/H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H3.1 and H3.2.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is expected to demonstrate broad species reactivity.
Species Reactivity
  • Mouse
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed. Uncharacterized bands may be observed at ~25 kDa and ~50 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Histone H3.1 and H3.2 in 10 μg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE154 04053252859960

Documentation

Anti-Histone H3.1/H3.2 Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Histone H3.1/H3.2 Antibody Certificates of Analysis

TitleLot Number
Anti-Histone H3.1/H3.2 - Q2147394 Q2147394
Anti-Histone H3.1/H3.2 - 3187403 3187403
Anti-Histone H3.1/H3.2 - 3321127 3321127
Anti-Histone H3.1/H3.2 - 3762589 3762589
Anti-Histone H3.1/H3.2 - 4074841 4074841
Anti-Histone H3.1/H3.2 - 4168776 4168776
Anti-Histone H3.1/H3.2 -2523883 2523883
Anti-Histone H3.1/H3.2 -2550068 2550068
Anti-Histone H3.1/H3.2 -2572998 2572998
Anti-Histone H3.1/H3.2 -2643616 2643616
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Technical Info

Title
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics