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MAB4212F Anti-HU-B27 Antibody, FITC conjugated

Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.

Anti-HU-B27 Antibody, FITC conjugated MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: MRAP, C21orf61, FGD2, FALP, B27, GCCD2 UniProt Number: P03989
MAB4212F
1 mL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RFC, ICC, IF, WBMFITCMonoclonal Antibody
Description
Catalogue NumberMAB4212F
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-HU-B27 Antibody, FITC conjugated
Alternate Names
  • Com-B27
Background InformationHU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
References
Product Information
FormatFITC
Control
  • Control IgG2a-FITC (MABC002F) as an isotype control
PresentationThe reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry.
Quality LevelMQ100
Applications
ApplicationDetect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Application NotesFlow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer.

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects/Fluorescence Intensity
Heterozygous HLA-B27+/Strong
Homozygous HLA-B7+/Weak
Heterozygous HLA-B7+/Weak - negative
Non B27, Non B7/Negative

This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.

SUGGESTED USAGE:
Flow cytometry and immunofluorescence - use 10 µl direct from the vial per 100 µl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 µl buffer.

Use Control IgG2a-FITC (MABC002F) as an isotype control.

Please note that this reagent should be used in a single-colour single-step assay only.
Biological Information
HostMouse
SpecificityThis reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F.
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Gene Symbol
  • MRAP
  • C21orf61
  • FGD2
  • FALP
  • B27
  • GCCD2
UniProt Number
Molecular Weight19 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot on Jurkat lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Com-B27 on 10 µg of Jurkat lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8ºC in the dark from date of receipt.
Packaging Information
Material Size1 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB4212F 04053252317200

Documentation

Anti-HU-B27 Antibody, FITC conjugated MSDS

Title

Safety Data Sheet (SDS) 

Anti-HU-B27 Antibody, FITC conjugated Certificates of Analysis

TitleLot Number
Anti-HU-B27, FITC conjugated 2470854
Anti-HU-B27, FITC conjugated - 2383541 2383541
Anti-HU-B27, FITC conjugated - 2430402 2430402
Anti-HU-B27, FITC conjugated - 2441930 2441930
Anti-HU-B27, FITC conjugated - 08051488828 08051488828
Anti-HU-B27, FITC conjugated - 08061492515 08061492515
Anti-HU-B27, FITC conjugated - 2109523 2109523
Anti-HU-B27, FITC conjugated - 2118021 2118021
Anti-HU-B27, FITC conjugated - 2295666 2295666
Anti-HU-B27, FITC conjugated - 2328355 2328355
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References

Reference overviewPub Med ID
Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3.
Trapani, J A, et al.
Hum. Immunol., 7: 205-16 (1983)  1982

Show Abstract 		6604046 6604046