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MABF2225-100UL Anti-HSV-2 VP16 Antibody, clone LP1

Anti-HSV-2 VP16, clone LP1, Cat. No. MABF2225, is a mouse monoclonal antibody that detects Alpha trans-inducing protein (VP16) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABF2225-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2225-100UL
DescriptionAnti-HSV-2 VP16 Antibody, clone LP1
Alternate Names
  • Herpes Simplex Virus VP16 Protein
  • Alpha trans-inducing protein
  • Alpha-TIF
Background InformationAlpha trans-inducing protein (UniProt: Q69113; also known as Alpha-TIF) is encoded by the VP16 (also known as UL48) gene (Gene ID: 1487335) in human Herpes Simplex virus 2. Herpes simplex virus (HSV) is a large enveloped DNA virus that replicates in the nuclei of mammalian cells. Its virion contains two regulatory proteins that are reported to modulate gene expression in infected cells: the transcriptional activator VP16 and the virion host shutoff protein vhs. VP16, an abundant structural protein, is shown to be essential for virion assembly and it stimulates transcription of the HSV immediate-early (IE) genes. Its transcriptional activation domain is localized in the C-terminal region. It is targeted to the TAATGARATTC consensus sequence found in IE promoters through interactions with the host factors Oct-1 and HCF. It is delivered by the infecting virion and acts during the earliest stages of infection to stimulate transcription of the viral IE genes, thereby facilitating the onset of the lytic program of viral gene expression. It is also reported to suppress vhs host protein synthesis and induce accelerated turnover of cellular and viral mRNAs. Clone LP1 is directed against VP16 in both HSV-1 and HSV-2. (Ref.: Smibert, CA., et al. (1994). J. Virol. 68(4); 2339-2346; McLean, C., et al. (1982). J. Gen. Virol. 63(2); 297-305).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-HSV-2 VP16, clone LP1, Cat. No. MABF2225, is a mouse monoclonal antibody that detects Alpha trans-inducing protein (VP16) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested Applications

Immunoprecipitation Analysis): A representative lot immunoprecipitated HSV-2 VP16 in Immunoprecipitation applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).

Western Blotting Analysis: A representative lot detected HSV-2 VP16 in Western Blotting applications (Crameri, M., et al. (2018). Nat Commun.; 9(1):1980; McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).

Immunofluorescence Analysis: A representative lot detected HSV-2 VP16 in Immunofluorescence applications (McLean, C., et al. (1982). J Gen Virol.; 63(2):297-305).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHerpes simplex virus -2; strain 25766.
EpitopeUnknown
CloneLP1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone LP1 is a mouse monoclonal antibody that detects VP16 protein of Herpes simplex virus 2 (HSV-2) and displays cross reactivity with HSV-1.
IsotypeIgG1
Species Reactivity
  • Herpes Simplex Virus
Species Reactivity NoteHerpes Simplex Virus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • VP16
Purification MethodProtein G purified
UniProt Number
Molecular Weight~57 kDa observed; 54.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HSV-1 infected Vero cells.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected VP16 protein in lysate from HSV-1 infected Vero cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2225-100UL 04065266908152