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MAB10209-I-25UL Anti-HSV-2 Nucleocapsid p40 Antibody, clone 3E1

Anti-HSV-2 Nucleocapsid p40, clone 3E1, Cat. No. MAB10209-I, is a mouse monoclonal antibody that detects HSV-2 Nucleocapsid p40 and is tested for use in Immunohistochemistry (Paraffin).

MAB10209-I-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMAB10209-I-25UL
DescriptionAnti-HSV-2 Nucleocapsid p40 Antibody, clone 3E1
Alternate Names
  • Capsid scaffolding protein
  • Capsid protein P40
  • Protease precursor
  • pPR
  • Virion structural protein UL26
Background InformationCapsid scaffolding protein (UniProt: P89449; also known as Capsid protein P40, Nucleocapsid protein P40, Protease precursor, pPR, Virion structural protein UL26) is encoded by the UL26 gene (Gene ID: 1487310) in Herpes Simplex virus 2 (HSV-2). HSV-2 is a large complex DNA-containing virus that is shown to induce the synthesis of at least 50 new polypeptides in infected cells. It replicates in the nuclei of mammalian cells. Its nuclear localization signal is localized in amino acids 428-431. Following its synthesis, Capsid protein 40 is processed into Assemblin (aa 1-237), and Assembly protein (aa 248-637) by proteolytic cleavage. Assemblin is a protease that plays an essential role in virion assembly within the nucleus. It exists in a monomer-dimer equilibrium with the dimer being the active species. It catalyzes the cleavage of the assembly protein after formation of the spherical procapsid. By that cleavage, the capsid matures and gains its icosahedral shape. The assembly protein is involved in capsid assembly. It acts as a scaffold protein by binding major capsid protein and multimerizes in the nucleus such as major capsid protein forms the icosahedral T=16 capsid. It is cleaved by Assemblin after capsid completion and the cleavages products are evicted from the capsid before or during DNA packaging. HSV is reported to evade the immune system through interference with MHC class I antigen presentation on the cell surface, by blocking the transporter associated with antigen processing (TAP) induced by the secretion of infected cell protein 47 (ICP-47) by HSV. ICP-47 prevents initiation of a CTL-response against HSV, allowing the virus to survive for a protracted period in the host. (Ref.: Goldsmith, K., et a. (1998). J. Exp. Med. 187(3); 341-348; Heilman, CJ., et al. (1981). J. Virol. 40(2); 508-515).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-HSV-2 Nucleocapsid p40, clone 3E1, Cat. No. MAB10209-I, is a mouse monoclonal antibody that detects HSV-2 Nucleocapsid p40 and is tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Biological Information
ImmunogenPurified HSV-2 nucleocapsids.
EpitopeUnknown
Clone3E1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 3E1 is a mouse monoclonal antibody that detects nucleocapsid protein p40 in Herpes Simplex virus 2
IsotypeIgG1
Species Reactivity
  • Herpes Simplex Virus
Species Reactivity NoteHerpes simplex virus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • UL26
Purification MethodProtein G purified
UniProt Number
Molecular Weight66.94 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in HSV-2-positive tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected HSV-2 Nucleocapsid p40 in HSV-2-positive tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB10209-I-25UL 04065267027777