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ABS2108-25UL Anti-HMG-Lysine

Anti-HMG-Lysine, Cat. No. ABS2108, is a rabbit polyclonal antibody that detects hydroxymethylglutaryl-lysine conjugated proteins and has been tested for use in Western Blotting.

Anti-HMG-Lysine MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling
ABS2108-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS2108-25UL
DescriptionAnti-HMG-Lysine
Alternate Names
  • 3-hydroxy-3-methyl-glutary-Lysine
Background InformationAcetylation of lysine residues in proteins has emerged as an important mechanism in regulation and coordination of normal and pathophysiological cell metabolism. Lysine HMGylation is a newer post-translational protein modification that occurs under normal basal conditions. In murine models of HMG-CoA lyase deficiency, higher levels of protein lysine HMGylation has been observed. This rabbit polyclonal antibody specifically detects chemically modified proteins containing hydroxymethylglutaryl-lysine modifications, but not unmodified proteins. (Ref.: Wagner, GR et al. (2017). Cell Metabolism 25(4); 823-837).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.1% sodium azide.
Applications
ApplicationAnti-HMG-Lysine, Cat. No. ABS2108, is a rabbit polyclonal antibody that detects hydroxymethylglutaryl-lysine conjugated proteins and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected HMG-Lysine in Western Blotting applications (Wagner, G.R., et. al. (2017). Cell Metab. 25(4):823-837; Anderson, K.A., et. al. (2017). Cell Metab. 25(4):838-855.e15).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected HMG-Lysine in HMG-BSA conjugate (Courtesy of Dr Kristin Anderson from Duke University).
Biological Information
Immunogen3-hydroxy-3-methyl-glutaryl (HMG)-Lysine conjugated to BSA.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects BSA-conjugated 3-hydroxy-3-methyl-glutaryl (HMG)-Lysine. It recognizes BSA chemically modified to contain HMG-lysine modifications, but not unmodified BSA.
IsotypeIgG
Species Reactivity
  • All
Species Reactivity NotePredicted to react with all species.
Antibody TypePolyclonal Antibody
Purification MethodAffinity Purified
Molecular Weight~75 kDa observed (BSA conjugate). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with HMG-BSA conjugate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected HMG-Lysine in HMG-Lysine-BSA conjugate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2108-25UL 04054839519369