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AB5472-50UL Anti-Glycine Receptor α3 Subunit Antibody

This Anti-Glycine Receptor α3 Subunit Antibody is validated for use in IH for the detection of Glycine Receptor α3 Subunit.

Anti-Glycine Receptor α3 Subunit Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: GLRA3 UniProt Number: O75311
AB5472-50UL
50 µL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5472-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Glycine Receptor α3 Subunit Antibody
Alternate Names
  • GLRa3
References
Product Information
FormatAffinity Purified
Control
  • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationThis Anti-Glycine Receptor α3 Subunit Antibody is validated for use in IH for the detection of Glycine Receptor α3 Subunit.
Key Applications
  • Immunohistochemistry
Application NotesWestern blot: 1:200 on rat brain membranes.

Immunohistochemistry on rat brain floating sections and mouse brain floating sections (trypsin pretreatment is recommended).

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenA purified peptide corresponding to amino acid residues 355-368 of rat GlyRa3 (Accession P24524).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Glycine receptor alpha3 (GlyRalpha3) protein. The epitope is not similar to any other known proteins.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • GLRA3
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O75311 # The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).
SIZE: 464 amino acids; 53800 Da
SUBUNIT: Pentamer composed of alpha and beta subunits (By similarity).
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely distributed throughout the central nervous system.
DOMAIN: SwissProt: O75311 The N-terminal domain carries structural determinants essential for agonist and antagonist binding. TM2 is thought to form the innert wall of the chloride channel. The cytoplasmic loop is an important determinant of channel inactivation kinetics.
SIMILARITY: Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
MISCELLANEOUS: The alpha subunit binds strychnine.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5472-50UL 04053252583711

Documentation

Anti-Glycine Receptor α3 Subunit Antibody MSDS

Title

Safety Data Sheet (SDS) 

Anti-Glycine Receptor α3 Subunit Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-GLYCINE RECEPTOR a3 (GlyRa3) -2696619 2696619
RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) - 4145788 4145788
RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) -2532320 2532320
RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) -2644610 2644610
RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) -3156746 3156746
RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) AFFINITY PURIFIED POLYCLONAL ANTIBODY 3116892
RABBIT ANTI-GLYCINE RECEPTOR α3 (GlyRα3) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2994766
RABBIT ANTI-GLYCINE RECEPTOR 3 (GlyR3) -2774103 2774103

References

Reference overviewPub Med ID
The human glycine receptor subunit alpha3. Glra3 gene structure, chromosomal localization, and functional characterization of alternative transcripts
Nikolic, Z, et al
J Biol Chem, 273:19708-14 (1998)  1998

9677400 9677400
Identification and functional expression of a novel ligand binding subunit of the inhibitory glycine receptor
Kuhse, J, et al
J Biol Chem, 265:22317-20 (1990)  1990

2176214 2176214

Data Sheet

Title
RABBIT ANTI-GLYCINE RECEPTOR 3 (GlyR3) AFFINITY PURIFIED POLYCLONAL ANTIBODY