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MABT51 Anti-Galectin-3 Antibody, clone M3/38

Anti-Galectin-3 Antibody, clone M3/38 is an antibody against Galectin-3 for use in WB, IP, IH & IC.

Anti-Galectin-3 Antibody, clone M3/38 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: LGALS3, L-31, GAL3, Galectin-3, GALIG, MAC2, GALBP, LGALS2, MAC-2, CBP35 UniProt Number: P16110
MABT51
50 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IP, IHC, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT51
DescriptionAnti-Galectin-3 Antibody, clone M3/38
Alternate Names
  • lectin, galactoside-binding, soluble, 3
  • Mac-2 antigen
  • Carbohydrate-binding protein 35
  • galectin-3
  • Galactose-specific lectin 3
  • Laminin-binding protein
  • Lectin L-29
  • IgE-binding protein
  • Galactoside-binding protein
Background InformationGalectin-3 belongs to the galectin gene family defined by the specificity of a carbohydrate recognition domain (CRD) for Gal/Lac and expression is usually elevated in a wide range of neoplastic cell types. The galectin CRD consists of 135 amino acids. Galectin-3 has been shown to effect tumor development and progression.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Galectin-3 Antibody, clone M3/38 is an antibody against Galectin-3 for use in WB, IP, IH & IC.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
  • Immunocytochemistry
Application NotesWestern Blot Analysis: 1:50,000 dilution from a previous lot detected Galectin-3 in 10 µg of NIH/3T3 cell lysate.

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Springer, T., et al. (1981). THE JOURNAL OF BIOLOGICACHLE MISTRY. 256(8):3833-3839.)

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH. (Gasbarri, A., et al. (1999). Journal of Clinical Oncology. 17(11):3494-3502.)

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (Gasbarri, A., et al. (1999). Journal of Clinical Oncology. 17(11):3494-3502.)
Biological Information
ImmunogenPlasma membrane glycoproteins corresponding to mouse Galectin-3.
CloneM3/38
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LGALS3
  • L-31
  • GAL3
  • Galectin-3
  • GALIG
  • MAC2
  • GALBP
  • LGALS2
  • MAC-2
  • CBP35
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity).

SUBUNIT STRUCTURE: Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.

SUBCELLULAR LOCATION: Nucleus. Note: Cytoplasmic in adenomas and carcinomas (By similarity). May be secreted by a non-classical secretory pathway and associate with the cell surface.

TISSUE SPECIFICTY: The highest levels are found in activated macrophages.

SEQUENCE SIMILARITIES: Contains 1 galectin domain.
Molecular Weight~28 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:50,000 dilution of this antibody detected Galectin-3 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT51 04053252290985

Documentation

Anti-Galectin-3 Antibody, clone M3/38 MSDS

Title

Safety Data Sheet (SDS) 

Anti-Galectin-3 Antibody, clone M3/38 Certificates of Analysis

TitleLot Number
Anti-Galectin-3, clone M3/38 2476943
Anti-Galectin-3, clone M3/38 2474212
Anti-Galectin-3, clone M3/38 - 2135135 2135135
Anti-Galectin-3, clone M3/38 - 1991862 1991862
Anti-Galectin-3, clone M3/38 - 2225189 2225189
Anti-Galectin-3, clone M3/38 - 2327014 2327014
Anti-Galectin-3, clone M3/38 - 2531557 2531557
Anti-Galectin-3, clone M3/38 - 3174817 3174817
Anti-Galectin-3, clone M3/38 - 3524845 3524845
Anti-Galectin-3, clone M3/38 - 3613019 3613019
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