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DR1067 Anti-GTF2I Mouse mAb (3E2)

Anti-GTF2I Mouse mAb (3E2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-General Transcription Factor IIi

DR1067
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~130 kDa GTF2I protein in HeLa cells and human colon and placenta tissue.
Catalogue NumberDR1067
Brand Family Calbiochem®
SynonymsAnti-General Transcription Factor IIi
Application Data
Detection of human GTF2I by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GTF2I by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (10 µg/ml). Detection: fluorescence.

Detection of human GTF2I by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.11.
Positive controlHuman colon tissue, Human placenta tissue, HeLa cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesELISA (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human GTF2l (aa 36-275), expressed as a GST fusion protein
Clone3E2
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1067 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-General Transcription Factor IIi
ApplicationELISA (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human GTF2I by immunoblotting. Sample: Whole tissue extract from human colon. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human GTF2I by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (10 µg/ml). Detection: fluorescence.

Detection of human GTF2I by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human placenta tissue. Primary antibody: Anti-GTF2I Mouse mAb (3E2) (Cat. No. DR1067) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~130 kDa GTF2I protein.
BackgroundGTF2l is required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
HostMouse
ImmunogenFull-length, recombinant, human GTF2l (aa 36-275), expressed as a GST fusion protein
Clone3E2
IsotypeIgG2a
Specieshuman
Positive controlHuman colon tissue, Human placenta tissue, HeLa cells
FormLiquid
FormulationIn PBS, pH 7.11.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review