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48-602MAG
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CB1015
Sigma-AldrichAnti-GTAP Rabbit pAb
Anti-GTAP, rabbit polyclonal, recognizes the ~67 kDa GTAP protein in HEK293 cells. It is validated for use in Western blotting.
More>>Anti-GTAP, rabbit polyclonal, recognizes the ~67 kDa GTAP protein in HEK293 cells. It is validated for use in Western blotting. Less<<
Anti-GTAP Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Due to a high degree of sequence identity, this antibody is also expected to react with mouse and rat GTAP. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
A synthetic peptide corresponding to amino acids 7-22 (QGQQQPGPGQQLGGQG) of human GTAP, conjugated to KLH.
Immunogen
Human
Host
Rabbit
Species Reactivity
Human
Mouse
Rat
Antibody Type
Polyclonal Antibody
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
03-June-2011 RFH
Synonyms
Anti-UBE2Q1 pAb
Application
Immunoblotting (3-10 µg/ml)
Application Data
Detection of human GTAP by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Primary antibody: Anti-GTAP Rabbit pAb (Cat. No. CB1015) (3-10 µg/ml). Detection: chemiluminescence.
Description
Protein A purified rabbit polyclonal antibody. Recognizes the ~67 kDa GTAP protein.
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). GTAP is a member of the E2 ubiquitin-conjugating enzyme family. GTAP may participate in intercellular adhesion and embryonic development. GTAP is thought to play a role in stem cell development via its regulation of laminin-dependent embryonic cell adhesion and the formation of embryoid bodies.
Host
Rabbit
Immunogen species
Human
Immunogen
A synthetic peptide corresponding to amino acids 7-22 (QGQQQPGPGQQLGGQG) of human GTAP, conjugated to KLH.
Species
human, mouse, rat
Form
Liquid
Formulation
In PBS.
Preservative
≤0.1% sodium azide
Comments
Due to a high degree of sequence identity, this antibody is also expected to react with mouse and rat GTAP. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).